Preparation method of sea squirt low molecular polypeptides

A low-molecular and sea squirt technology, applied in the direction of fermentation, can solve the problems of a large amount of fresh water and energy, which do not conform to the mainstream, and achieve the effect of improving extraction rate and purity, reducing production cost, and reducing production energy consumption

Inactive Publication Date: 2013-12-04
NINGBO BOFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hot water extraction method needs to use a large amount of fresh water and energy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0008] Example 1: After soaking and cleaning raw sea squirts, first use a pulverizer to pulverize them into a fluid slurry, then enter an industrial-grade colloid mill for ultrafine pulverization to a particle size of about 30 microns, and mix with 4 times the volume of water evenly Enter the high-speed centrifuge at 12,000 rpm and continuously centrifuge for 30 minutes to remove non-collagen and other water-soluble substances of active substances. The sediment containing sea squirt polypeptide and 2 times the volume of water enter the biological enzymatic hydrolysis tank for compound enzymatic hydrolysis treatment. The compound enzyme is mainly composed of pepsin, papain and collagenase, the added amount is 0.2% of the raw material, the reaction conditions are temperature 40°C, pH 8.0, and reaction time 2.5 hours. The enzymatic hydrolysis product is treated with ultrafiltration equipment. The permeation molecular weight of the ultrafiltration membrane is 2KD. Those with a mole...

example 2

[0009] Example 2: After soaking and cleaning the sea squirt as a raw material, first use a pulverizer to pulverize it into a fluid slurry, then enter an industrial-grade colloid mill for ultrafine pulverization to a particle size of about 50 microns, and mix it with 2 times the volume of water evenly Enter the high-speed centrifuge at 8000 rpm for continuous centrifugation for 30 minutes to remove non-collagen water-soluble substances, and the collagen-containing precipitate and 5 times the volume of water enter the biological enzymatic hydrolysis tank for compound enzymatic hydrolysis treatment. The compound enzyme is mainly composed of trypsin, papain and collagenase, the added amount is 2% of the raw material, the reaction conditions are temperature 50°C, pH 7.5, and reaction time 4.5 hours. The enzymatic hydrolysis product is treated with ultrafiltration equipment. The permeation molecular weight of the ultrafiltration membrane is 2KD. Those with a molecular weight less tha...

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Abstract

The invention discloses a preparation method of sea squirt low molecular polypeptides. The preparation method comprises the following steps: immersing and cleaning sea squirts, crushing the sea squirts by using a crusher, adding to an industrial grade colloid mill, carrying out ultramicro crushing to a particle size of 30-50mum, uniformly mixing with water having a volume 2-6 times the volume of obtained particles, adding to a centrifuge, continuously centrifuging at 8000-12000rpm for 30min, removing the obtained supernatant, adding the obtained precipitate and water having a volume 2-6 times the volume of the obtained precipitate to a biological enzymatic hydrolysis pot, carrying out enzymatic hydrolysis under conditions comprising a temperature of 37-55DEG C and a pH value of 7.5-8.0 for 2-6h, processing the obtained enzymatic hydrolysis product by a 2KD ultrafilter membrane, carrying out nanofiltration membrane concentration of the part having a molecular weight below 2KD in the product, and carrying out spray drying to obtain the finally-finished sea squirt low molecular polypeptides, wherein the addition amount of a composite enzyme in the above enzymatic hydrolysis reaction is 0.2-2% of the amount of a raw material. The part having a molecular weight above 2KD in the enzymatic hydrolysis product returns to the biological enzymatic hydrolysis pot for enzymatic hydrolysis.

Description

technical field [0001] The invention belongs to a method for extracting seafood, in particular to a method for extracting and preparing sea squirt polypeptides. technical background [0002] Sea squirts belong to the phylum of chordates, and they belong to the class Urochordate. There are about 1,250 species in the world. They are distributed in intertidal zones, open seas and deep seas. They have remarkable characteristics in many aspects such as growth, reproduction, metabolism, and adaptation mechanisms. There are abundant natural resources of sea squirts in my country, and 103 species have been discovered so far, including 5 species in the Bohai Sea, 21 species in the Yellow Sea, 24 species in the East China Sea, and 53 species in the South China Sea. Due to the special status of sea squirts in evolution, it has received high attention from zoologists, evolutionists and endocrinologists, and has become a research and development hotspot. Since the 1980s, many physiologic...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 张建芳
Owner NINGBO BOFENG BIOTECH
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