Open-type constant-current diffusion protein crystallization array chip and manufacturing method thereof

Abstract

The invention relates to an open-type constant-current diffusion protein crystallization array chip and a manufacturing method thereof, which are particularly suitable for biology process study and new drug design. The open-type constant-current diffusion protein crystallization array chip comprises a basement and a substrate, wherein a plurality of protein crystallization units are arranged on the substrate; circular micro-flow main channels are arranged on the combined plane of the substrate and the basement, communicated with the protein crystallization units through micro-flow diffusion channels, and communicated in series with one another in sequence; the circular micro-flow main channels and the protein crystallization units in the middle thereof form a single-line crystallization cell array, or a plurality of single-line crystallization cell arrays are connected in parallel to form a multi-line crystallization cell array. The open-type constant-current diffusion protein crystallization array chip and the manufacturing method have the advantages that the protein crystallization speed as well as the crystal clustering density is high; a protein crystal formed in a crystallization unit is free of the action of shearing force, and can be extracted and analyzed conveniently; the protein consumption is low; the operation is flexible; suitability for high-pass screening of crystallization conditions is realized; the chip is convenient to manufacture; the structure is compact; the cost is low; mass production is realized.

Description

Technical field [0001] The present invention involves an open constant current diffusion protein crystalline array chip and its production method. It is a device that directly, effective and comprehensively understanding the protein advanced structure and functions through protein crystals.It belongs to the field of biotechnology. Background technique [0002] The study of protein structure and function is of great significance for understanding the life process and new drug design at the molecular level.The crystal analysis of protein is the most direct and effective way to fully understand the three -dimensional structure of the protein, which has received widespread attention from people. [0003] In order to efficiently obtain protein crystals that can be used for crystal parsing, a small amount of protein solution needs to be used for reasonable conditioning (such as protein solution concentration, sediment concentration, pH of solution, temperature, buffer solution type, et...

AI Technical Summary

Problems solved by technology

[0008] A common problem in the above experiments is that when the volume of the liquid is constant, the total amount of protein molecules contained is very limited, and it is difficult to promote the crystallization of protein molecules

If the concentration of protein is increased, the crystallization efficiency can be increased, but the obtained protein crystals are small in size and low in cluster density, so it is difficult to be directly used for diffraction analysis

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