Method for preparation of hydroxyapatite nanorod and nanowire by hydrothermal process

A hydroxyapatite and nanorod technology, applied in chemical instruments and methods, nanotechnology, nanotechnology, etc., can solve the problems of increasing process complexity, production cost, environmental pollution, etc., and achieve excellent protein adsorption and release performance, Environmentally friendly, shape-controllable effects

Active Publication Date: 2015-05-13
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The surfactants and organic or inorganic templates added in this method need to be removed in subsequent processing, thus increasing the complexity of the process and production costs, and causing environmental pollution

Method used

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  • Method for preparation of hydroxyapatite nanorod and nanowire by hydrothermal process
  • Method for preparation of hydroxyapatite nanorod and nanowire by hydrothermal process
  • Method for preparation of hydroxyapatite nanorod and nanowire by hydrothermal process

Examples

Experimental program
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Embodiment 1

[0033] 10 mL of 0.1M calcium chloride aqueous solution was added dropwise to 20 mL of 15.5 mg / mL aqueous solution of riboflavin sodium phosphate at a rate of 10 mL / min. The pH of the mixture was adjusted to 9 with 1M sodium hydroxide, and stirred for 5 minutes. The mixed solution was placed in a hydrothermal reactor, the hydrothermal heating temperature was 180°C, and the reaction was performed for 24 hours. After the reaction, the precipitate was collected by centrifugation, washed with water, and dried in the air at 60°C to obtain hydroxyapatite nanorods.

[0034] The samples were characterized by SEM and TEM, such as figure 1 and 2 shown. It can be seen from the figure that the morphology of the prepared samples is nanorods, the length of the nanorods is about 50 nanometers to 200 nanometers, and the width is about 20 nanometers to 50 nanometers. The obtained hydroxyapatite nanorods have the characteristics of good dispersibility and uniform size.

Embodiment 2

[0036] 10 mL of 0.1M calcium chloride aqueous solution was added dropwise to 20 mL of 15.5 mg / mL aqueous solution of riboflavin sodium phosphate at a rate of 15 mL / min, the pH of the mixture was adjusted to 5 with dilute hydrochloric acid, stirred for 5 minutes, and The mixed solution was placed in a hydrothermal reactor, the hydrothermal heating temperature was 180°C, and the reaction was performed for 24 hours. After the reaction, the precipitate was collected by centrifugation, washed with water, and dried in the air at 60°C to obtain hydroxyapatite nanowires.

[0037] The samples were characterized by SEM and TEM, such as image 3 and 4 shown. It can be seen from the figure that the morphology of the prepared samples is nanowires, and the length of the nanowires is several micrometers to tens of micrometers.

Embodiment 3

[0039] The hydroxyapatite nanorods and nanowires obtained in Example 1 and Example 2 were added to an aqueous solution containing bovine hemoglobin, and after shaking at 37 ° C for 24 hours, the product was centrifuged, washed, and dried to obtain adsorbed protein. of hydroxyapatite nanorods / nanowires. The protein-adsorbed hydroxyapatite nanorods / nanowires exhibited good sustained-release performance and pH-controlled release behavior in phosphate buffers with different pH values. like Figure 5 As shown, in phosphate buffer at pH 4.5, the hydroxyapatite nanorods and nanowires adsorbed with bovine hemoglobin reached equilibrium for 12 hours, and the release amounts were 7% and 12%, respectively; however, in phosphate buffer at pH 7.4 The hydroxyapatite nanorods and nanowires adsorbed with bovine hemoglobin in the buffer reached equilibrium after 142 hours, and the equilibrium release amounts were 44% and 36%, respectively. This is because bovine hemoglobin is a neutral prote...

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Abstract

The invention relates to a method for preparation of a hydroxyapatite nanorod and a nanowire by a hydrothermal process. The method includes: adding a water soluble calcium salt solution into a riboflavin sodium phosphate solution at a rate of 3-50ml / min, adjusting the pH of the obtained mixed solution to 8-12 or 4-6, then leaving the solution to undergo a hydrothermal reaction at 100-200DEG C for 1-48h, carrying out separation, washing and drying the precipitate, thus obtaining the hydroxyapatite nanorod and nanowire.

Description

technical field [0001] The invention relates to a method for preparing hydroxyapatite nanorods and nanowires by a hydrothermal method. In particular, it relates to a method for preparing hydroxyapatite nanorods and nanowires by using an organic biomolecule riboflavin sodium phosphate as a phosphorus source and an inorganic calcium salt as a calcium source by a hydrothermal method, which belongs to the field of biological material preparation and drug sustained release . Background technique [0002] As the main inorganic component of hard tissues in vertebrates, hydroxyapatite has the advantages of excellent biocompatibility and biodegradability, as well as natural non-toxicity and not easy to be phagocytosed by immune cells. Therefore, hydroxyapatite materials are ideal Inorganic biomaterials. The nanostructured hydroxyapatite material has a high specific surface area and thus a high drug loading. Calcium ions on the surface of hydroxyapatite materials have good complexa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B25/32B82Y30/00
Inventor 朱英杰赵新宇陈峰
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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