Molecular marker for Y-type high molecular weight glutelin subunit gene in distant hybridization generation of wheat and aegilops sharonensis and application of molecular marker

Abstract

The invention provides a molecular marker for a Y-type high molecular weight glutelin subunit (HMW-GS) gene in a distant hybridization generation of wheat and aegilops sharonensis, wherein the molecular marker is obtained by amplifying a primer with a nucleotide sequence shown in SEQ ID NO.1-2. PCR (polymerase chain reaction) is carried out by utilizing the primer, so whether the distant hybridization generation of wheat and aegilops sharonensis contains an aegilops sharonensis Y-type HMW-GS gene locus or not can be rapidly and accurately detected, and whether the distant hybridization generation of wheat and aegilops sharonensis contains a Y-type HMW-GS gene or not is judged. By adopting the molecular marker method, whether the aegilops sharonensis Y-type HMW-GS gene transfers or exists in a wheat hybrid can be accurately detected, the aegilops sharonensis Y-type HMW-GS gene can be conveniently tracked, breeding working efficiency is greatly improved, a wheat quality breeding course is sped up, and the molecular marker for the Y-type HMW-GS gene in the distant hybridization generation of wheat and aegilops sharonensis has a good application prospect in the field of wheat crossbreeding.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to the molecular marker and the The application thereof is a specific primer for amplifying molecular markers in a PCR method, and the invention also relates to identification of progeny hybrids of distant hybridization of wheat and selection of varieties (lines) by using the primers. Background technique [0002] In the process of genetic improvement of wheat, specific beneficial genes of wild relatives of wheat can be introduced into common wheat through distant hybridization, thereby changing the genetic composition of wheat and effectively improving the agronomic traits of wheat. Therefore, it is widely used in wheat breeding. As a wild relative of wheat, Goatwort species have excellent resistance to stripe rust, leaf rust, stem rust, powdery mildew, drought resistance, salt and alkali resistance, cold resistance, high protein, etc. Control disease resistance, stress resistance...

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Problems solved by technology

[0005] However, although morphological identification is intuitive, it has considerable limitations due to the influence of subjective factors such as experience and the fact that some target traits cannot be expressed through morphology; while cytological identification and in situ hybridization must be cultivated into plants by hybrids. The season and time of material collection are strict, and the analysis and identification methods are complicated, requiring high technology, operation and practical experience, and because the identification is carried out at the chromosome level, due to the exchange and loss of chromosome fragments in the process of cell division, it is very difficult It is difficult to determine whether the target trait gene actually exists and is expressed normally

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