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Molecular Identification Method of Cotton Null Fruiting Branch Gene

A zero-type fruit branch and molecular identification technology, applied in the biological field, can solve the problems of speeding up the breeding and improvement of zero-type fruit branch products, poor accuracy, and long time, so as to speed up the process of breeding and improvement, with high accuracy and speed fast effect

Active Publication Date: 2016-06-01
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems in the prior art that the field identification and detection method of cotton strains containing the zero-type fruiting branch gene takes a long time, consumes a lot, and has poor accuracy, and provides a molecular identification method for the zero-type fruiting branch gene. Thereby, the breeding and improvement process of zero-type fruiting branch strains can be accelerated

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  • Molecular Identification Method of Cotton Null Fruiting Branch Gene
  • Molecular Identification Method of Cotton Null Fruiting Branch Gene

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Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with the examples.

[0037] Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased.

[0038] Tris-Cl: Tris(hydroxymethyl)aminomethane-HCl; EDTA: Ethylenediaminetetraacetic acid; SDS: Sodium dodecylsulfate; PVP: Polyvinyl Polyvinylpyrrolidone

[0039] All reagents were purchased from: Sangon Bioengineering (Shanghai) Co., Ltd.; purity: analytically pure.

[0040] 1 DNA extraction

[0041] A total of 32 mat...

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Abstract

The invention belongs to the field of biotechnology, relates to a molecular identification method for zero-type fruit branches of cotton, and particularly provides a gene specific primer marker used for identifying a cotton strain with zero-type fruit branch genes. Nucleotide sequences are as shown in SEQ ID No.1 and SEQ ID No.2. An individual strain carrying the zero-type fruit branch genes in a segregation population is identified by the gen specific marker. The method is high in identification speed, high in accuracy, simple to carry out and very suitable for indoor identification and molecular marker-assisted selection of the zero-type branches of the cotton.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular identification method for zero-type fruit branch gene identification and molecular marker-assisted breeding. Background technique [0002] Zero-type fruiting branch refers to a mutation character in which the fruiting branch in cotton is limited. The performance is that the bolls are directly born in the axils of the main stem or leaves, or there is only one fruit node. Due to its growth characteristics, the zero-type fruit branch material basically stops vegetative growth after cotton topping, which can reduce the labor required for cotton pruning management. At the same time, because most of the nutrients are supplied to the growth and development of cotton bolls, cotton bolls develop quickly and mature early, and the bolls in the upper and lower parts of a single plant have better maturity consistency, which is beneficial to cotton harvesting. The cotton ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈伟张永山姚金波李燕褚丽刘海菊
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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