Detection method of goat CMTM2 gene insertion/deletion polymorphism and application thereof

Abstract

The invention discloses a detection method of goat CMTM2 gene insertion / deletion polymorphism and an application thereof. According to the invention, by using the whole genome DNA of the goat to-be-tested as a template, a partial fragment of the goat CMTM2 gene is amplified by PCR, and then agarose gel electrophoresis is carried out, According to the electrophoresis results, the genotype at a 14-bp insertion / deletion polymorphism site of the goat CMTM2 gene NC_030825.1: g.35582961_35582974 site is identified. Correlation analysis shows that the different genotypes of 14-bp insertion / deletion polymorphism of the goat CMTM2 gene and the property of litter size of Shanbei white cashmere goats have a significant correlation, and DNA markers capable of increasing the property of litter size areexisted. The method can be applied to goat molecular marker-assisted selection breeding, and accelerates the establishment of an excellent goat genetic resource group.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the rapid and accurate typing detection of the 14-bp insertion / deletion polymorphism (InDel) site of goat CMTM2 gene NC_030825.1:g.35582961_35582974 and its molecular marker assisted selection. (MAS) applications in breeding. Background technique [0002] In recent years, with the rapid development of biotechnology, people have used advanced biotechnology and information technology means such as high-throughput sequencing and molecular markers to establish a platform that combines conventional breeding and molecular breeding, enabling breeding work to be transformed from experience to experience. A fundamental change in science has greatly improved breeding efficiency. Since 1950, great progress has been made in livestock and poultry breeding. The quantitative genetic theory has gradually formed the main breeding method, which has been applied to animal breeding...

AI Technical Summary

Problems solved by technology

InDel mostly focuses on the genome research of humans and various crops (such as rice and corn, etc.), and on livestock and poultry, it focuses on the research on chicken growth traits, and there is little research and application on ruminants

However, there is no report on the InDel locus of CMTM2 gene and its significant correlation with litter size traits

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