Method for cultivating haematococcus pluvialis in large scale and producing natural astaxanthin seasoning packet by haematococcus pluvialis

Abstract

The invention provides a method for cultivating haematococcus pluvialis in a large scale and producing a natural astaxanthin seasoning packet by haematococcus pluvialis. The method provided by the invention comprises a cultivation phase and an astaxanthin production phase. The method for cultivating the haematococcus pluvialis in large scale and producing the natural astaxanthin seasoning packet by the haematococcus pluvialis is reliable, stable in process and developed in technology, and provides powerful technical supports for popularization and applying of the deep processing of the haematococcus pluvialis and a natural astaxanthin product thereof; meanwhile, the method provides a use reference effect to the cultivation of other microalgae and propels the whole microalgae cultivation industry to grow rapidly and healthily.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation and its deep processing, in particular to large-scale cultivation of Haematococcus pluvialis and a method for producing natural astaxanthin seasoning packs. Background technique [0002] Haematococcus pluvialis belongs to Algae, Chlorophyceae, Volvox, Haematococcus, Haematococcus genus, Pluvialls species; the growth of the whole Haematococcus pluvialis is divided into green algae stage and red algae stage, In the stage of green algae, under the conditions of suitable light, temperature, nutrition, pH value, etc., the algae cells will grow at the rate of 2 n (n=1~4) geometric multiplication, can reach 5.0x10 5 ~1.0x10 6 pc / ml, the diameter of green algae cells is 10~20um, there is almost no accumulation of astaxanthin in algae cells at this stage; , the green algae turns into the red algae stage, and the algal cells gradually transform into static sporophytes (in other words, cys...

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Problems solved by technology

In small-scale experiments and production, physical methods (steam, filtration) or chemical methods (sodium hypochlorite) can be used to sterilize and disinfect the culture medium and containers to kill harmful species. In the process of Haematococcus, the culture medium is from several tons to hundreds of tons, and the culture container is also gradually enlarged; if the physical method (steam) is used again, a lot of energy will be wasted for related heating and cooling. Increased production costs are not conducive to promotion and realization; if the physical method (filtration) is used again, the theoretical value of the effective efficiency of various filtering equipment or instruments can only reach 99.9%. It will bring immeasurable losses to production, and with the prolongation of the frequency and time of use, the treatment effect cannot meet the requirements again; if chemical methods (sodium hypochlorite) are used again, a large amount of neutralizing chemicals must be consumed, which not only increases The cost is also due to the use of neutralizing agents, which have an immeasurable impact on the proliferation and accumulation of Haematococcus pluvialis

[0007] When Haematococcus pluvialis encounters adversity, a large number of sporophytes (red algae stage) accumulate natural astaxanthin, the algal cells become larger (40-60um), and the cell wall (pectin layer and cellulose layer) thickens, and now Some commonly used techniques cannot break the wall, and finally the algae powder produced cannot extract natural astaxanthin

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