Analysis method of volatile component in vanilla bean crude drug

Abstract

The invention discloses an analysis method of a volatile component in a vanilla bean crude drug. According to the analysis method, the vanilla bean crude drug is taken as a material; firstly, the volatile component of vanilla beans is extracted by adopting headspace solid-phase micro extraction; the volatile component is analyzed by adopting a gas chromatography-mass spectrometry method; the percent content of each component is calculated from a total ion chromatogram by data treatment by a chemical work station and by using an area normalization method; retrieval is carried out by a spectral library of the NIST (national institute of standards and technology) according to a mass spectrum of each peak; check with document literature is carried out according to a mass spectrometry lobe diagram of each peak, so as to determine each component. By adopting the analysis method, the volatile component in the vanilla bean crude drug is extracted and analyzed by adopting the headspace solid-phase micro extraction combined with the gas chromatography-mass spectrometry method for the first time; the defects of incomplete extraction of the volatile component when the traditional extraction method is used are compensated; a relatively comprehensive detection method is provided for separation and analysis of the volatile component of the crude drug.

Description

technical field [0001] The invention relates to an analysis method for chemical components in medicinal materials, in particular to an analysis method for volatile components in vanilla bean medicinal materials. Background technique [0002] Vanilla bean is the mature fruit of Vanilla planifoli Andrews, a plant of the genus Vanilla in the family Orchidaceae. It is native to Madagascar, an island country in Africa, and has been introduced and cultivated in Xishuangbanna, Yunnan, my country and other places. Vanilla beans have the functions of relieving wind and spasm, calming and anticonvulsion. Modern studies have shown that it also has pharmacological activities such as sedative, anticonvulsant, antioxidative, and antibacterial. Its main chemical components are aldehydes, phenols, terpenes, esters, volatile oils, sugars, and organic acids. Vanilla bean is a precious natural food spice, which is widely used in medicine, food and other fields because of its plump and rich ar...

AI Technical Summary

Problems solved by technology

However, if the distillation temperature of the SDE method is too high, the sample may be hydrolyzed, oxidized, esterified or thermally decomposed, and the components with high boiling points are difficult to distill out together with the water vapor.

Therefore, neither of them can fully reflect the true composition of the volatile components in the sample.

[0004] In addition, due to the differences in vanilla varieties, origins and processing methods, the composition and content of volatile components are different, so it is difficult to make a comprehensive and objective evaluation of the quality of vanilla beans.

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