Method for detecting clamp connection in edible fungi mating type research

A lock joint and mating type technology, which is applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of relatively large influence on the observation results of the insert position and the inconspicuous lock joint, and achieve good application Foreground, ease of operation, and the effect of improving the confidence interval

Inactive Publication Date: 2013-11-13
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the methods used are inserting method (see "Research on Mating Type of Black Fungus" 2002 for details). For the edible fungus such as tree flower whose locking joints are not very obvious and appear in very special pa...

Method used

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  • Method for detecting clamp connection in edible fungi mating type research
  • Method for detecting clamp connection in edible fungi mating type research
  • Method for detecting clamp connection in edible fungi mating type research

Examples

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Embodiment 1

[0021] 1. Culture medium production:

[0022] 1) Peel the potatoes and weigh 200g / L, cut into 2mm slices and immerse in quantitative distilled water;

[0023] 2) Bring to a boil over high heat, then simmer for 1 hour on low heat. (It needs to be stirred at the beginning to prevent sticking to the bottom);

[0024] 3) Place on ice to cool the precipitate, then vacuum filter;

[0025] 4) Quantitative, sub-package to 500ml / bag (different specifications can be sub-packaged according to needs), and store in the refrigerator at -20°C;

[0026] 5) Melt in hot water (50°C) when used, and vacuum filter again to obtain potato extract;

[0027] 6) Add 100ml of the above potato extract, add 2g of glucose, 15g of agar powder, 2ml of Tween (Tween) 80, distilled water to 1L;

[0028] 7) The above-mentioned culture medium was sterilized at 121°C for 20 minutes, and dispensed into disposable plates with a dispenser, 10 ml per plate.

[0029] 2. Affinity reaction observation:

[0030] Ino...

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Abstract

The invention relates to a method for detecting clamp connection in edible fungi mating type research. The method comprises the steps of preparing a special culture medium; sterilizing the culture medium; separately filling the culture medium into a disposable flat plate by using a liquor separator; closely and pairwise inoculating tested mononuclear strains to the center of the flat plate, placing in a culture room at temperature of 25-26 DEG C for culture; observing whether the clamp connection exists in a hypha growth area or not after 7-8 days. The method disclosed by the invention can very accurately judge whether the clamp connection exists or not, thereby enlarging the confidence interval of experimental research; the method disclosed by the invention is easy and convenient to operate, omits the complex steps of selecting hypha in a junction area, culturing the hypha, inserting slices and carrying out microscopic examination on the hypha inserted with slices in the conventional method, reduces the anthropogenic influence factors in a test to minimum, saves the time and has good application prospect.

Description

technical field [0001] The invention belongs to the field of edible fungus breeding, and in particular relates to a method for detecting lock joints in research on mating types of edible fungi. Background technique [0002] The study of the mating type system of edible fungi is an important genetic basis for the breeding of edible fungi. The research method is to obtain the spore monokaryon of the test strain and then carry out the test reaction of pairwise mating, and observe the presence or absence of lock joint under the optical microscope to judge the affinity The main basis for the reaction. Therefore, it is the most critical part of this experimental research to observe the presence or absence of lock-like union accurately, effectively and quickly. At present, most of the methods used are inserting method (see "Research on Mating Type of Black Fungus" 2002 for details). For the edible fungus such as tree flower whose locking joints are not very obvious and appear in ...

Claims

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Application Information

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IPC IPC(8): G01N21/84
Inventor 王瑞娟尚晓冬张美彦周峰
Owner SHANGHAI ACAD OF AGRI SCI
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