Preparation method for biological microbial protease preparation

A technology of protease preparation and biological bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of short active storage period, single strain, low survival rate, etc., to increase production and increase production and preservation rate, increase the effect of growth rate

Inactive Publication Date: 2013-11-13
湖北宏全生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the above problems, the present invention designs a preparation method of biological bacterium protease preparation, which solves the defects of various existing microbial preparations such as single species, low survival rate, poor stability, short active storage period and easy autolysis. , greatly increase the production and preservation rate of microbial agents, as livestock feed additives, not only can prevent and resist diseases, but also can increase production and increase growth rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A preparation method for biological bacterium protease preparation, comprising the following steps:

[0017] (1) Bacillus subtilis (Bacillus subtilis) bacterial liquid, brewer's yeast (Sac-charomyces cerevisiae), Delbrueckii (L. Cultivate in the lactic acid bacteria culture solution, and collect the bacteria respectively;

[0018] (2) Mix the thallus prepared in step (1) with bran, isomaltooligosaccharide, vegetable protein, and chitin, then add water twice the total mass of the raw materials, stir, mix evenly, then dry, and after making pellets to obtain the product.

[0019] Wherein, the mixing weight ratio of the Bacillus subtilis thallus, brewer's yeast thalline, Lactobacillus delbrueckii thallus, bran, isomaltose oligosaccharide, vegetable protein, and chitin is 3: 2: 1: 50: 10: 5:2.

[0020] The preparation method of the Bacillus subtilis thallus is as follows: the required culture medium is 15.0g of peptone, 0.50g of beef extract, 20.0g of glucose, 5.0g of sod...

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Abstract

The invention discloses a preparation method for a biological microbial protease preparation. The preparation method comprises the following steps: culture and collection of thalli; material preparation and granulation; etc. The invention has the following advantages: the preparation method overcomes the defects of a single strain, a low survival rate, poor stability, a short activity retention period, easy occurrence of autolysis, etc. of a variety of conventional microbial preparations and greatly improves the output and the preserving rate of microbial inocula; and as a feed additive for livestock, the prepared biological microbial protease preparation can prevent and resist diseases, improve the output of the livestock and increase the growth speed of the livestock.

Description

technical field [0001] The invention relates to a protease, in particular to a method for preparing a biological bacterium protease preparation, which belongs to the field of microbial preparations. Background technique [0002] A microbial agent is a microbial preparation that replaces or balances one or more bacterial strains in an animal's ecosystem. In a narrow sense, it is a microbial product that can stimulate the growth of its own beneficial bacteria while resisting the growth of harmful bacteria. Microbial preparations contain a large number of beneficial microorganisms such as lactic acid bacteria and yeast, which can multiply rapidly after entering the livestock and poultry as feed. On the other hand, a normal microbial flora is formed in the host body, which synthesizes major vitamins for the host, provides nutrition and prevents the invasion of pathogenic bacteria. At present, there are two main types of microbial preparations on the market: powder and water. H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12N1/14C12R1/125C12R1/865C12R1/225
Inventor 马振宇王洪全曾宪梅王婕
Owner 湖北宏全生物科技有限公司
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