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HIV (human immunodeficiency virus) antibody recognition reagent

A technology of human immunodeficiency and antibodies, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation and long reaction time, and achieve the effect of simple operation, simple operation and avoiding mistakes

Inactive Publication Date: 2013-10-30
齐明山 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only a few companies producing WB at home and abroad, and the response time is long and the operation is cumbersome.

Method used

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  • HIV (human immunodeficiency virus) antibody recognition reagent
  • HIV (human immunodeficiency virus) antibody recognition reagent
  • HIV (human immunodeficiency virus) antibody recognition reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Manufacture of HIV Antibody Confirmation Reagent

[0040] 1. Main materials

[0041] 1.1 HIV-specific antigens are gp160, gp120, p66, p55, p51, gp41, p31, p24, p17 of HIV-1 and gp36 of HIV-2: provided by Beijing Academy of Military Medical Sciences, HIV antigen expressed by genetic engineering; human IgG And mouse anti-human IgG: provided by Shenzhen Faipeng Biotechnology Co., Ltd.; NC membrane: product of Millipore Company of the United States; bovine serum albumin (BSA), polyethylene glycol (PEG) 20000, hydrolyzed protein: product of Sigma Company of the United States; others Reagents are chemical reagents of routine analytical grade.

[0042] 1.2 Testing instrument: Immunochromatographic knot reading recorder, model: NS3001, product of Tianjin Zhongxin Keju Biopharmaceutical Co., Ltd.

[0043] 1.3 Clinical serum: Obtained by the company in relevant hospitals, 19 HIV-1 positive samples and 1 HIV-2 positive sample have been confirmed by Singapore MP reagen...

Embodiment 2

[0063] Embodiment 2: clinical trial

[0064] 1. Main materials

[0065] 1.1 Examination of human immunodeficiency virus antibody confirmation reagents: the reagents were optimized and prepared according to Example 1.

[0066] 1.2 Confirmation reagent for control human immunodeficiency virus antibody: product of Singapore MP Company.

[0067] 1.3 Clinical samples: Obtained by the company in the relevant infection hospital, 100 HIV-positive samples, including 95 HIV-1 positive samples, positive for HIV antibody in ELISA preliminary screening; 5 HIV-2 positive samples, confirmed positive samples; 500 negative samples The samples were collected from healthy people, and the comprehensive clinical test was judged to be negative.

[0068] 2. Detection method

[0069] Carry out according to the method of the respective product instructions, determine the test results, and conduct analysis.

[0070] 3. Results: The test results are as follows:

[0071] Table 3: Comparison of test ...

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Abstract

The invention relates to an HIV (human immunodeficiency virus) antibody recognition reagent. The HIV antibody recognition reagent comprises a piece of HIV antibody test paper, a washing solution, a developing system, a positive control substance, a negative control substance and an immunochromatographic result interpretoscope; the HIV antibody test paper is coated with 10 protein stripes including a HIV1 gp160 stripe, a gp120 stripe, a P66 stripe, a p51 stripe, a gp41 stripe, a p24 stripe, a p17 stripe, a HIV2 gp36 stripe and two human IgG (immunoglobulin G) stripes with different concentrations; and the developing system comprises a collaurum or biotin-labeled anti-human IgG or SPA and a biotin-labeled avidin. During testing, a sample is put on the HIV antibody test paper, unrelated proteins are removed by washing, the stripes are developed by using the developing system, and the result is judged with the immunochromatographic result interpretoscope or naked eyes according to the developing condition of the stripes. The HIV antibody recognition reagent provided by the invention is easy and convenient to operate, is suitable for field testing and is economic, practical and the like.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to an in vitro diagnostic reagent used for a human immunodeficiency virus antibody confirmation reagent. Background technique [0002] Since AIDS, caused by the human immunodeficiency virus (HIV), was discovered in the 1980s, it has spread worldwide. According to the United Nations AIDS Agency (UNAIDS) report, by the end of 2010, the number of people infected with AIDS in the world reached 37 million. There are currently more than 700,000 HIV-infected people in my country. So far, there are no effective drugs and vaccines to treat and prevent AIDS, so HIV infection has become the most serious infectious disease and public health problem in the world today. Because of this, the monitoring of HIV infection has been highly valued by countries all over the world. In 1985, WHO, the US FDA and many developed countries have successively formulated regulations on the scree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 齐明山吴澜
Owner 齐明山
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