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Liquid-phase chip kit for detection of acute coronary syndrome based on serum exosomes and preparation method

A liquid phase chip and kit technology, applied in the field of in vitro kits, can solve the problems of low specificity of related kits, and achieve the effects of simple preparation method, high detection efficiency and high sensitivity

Inactive Publication Date: 2013-10-30
梁春 +2
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Problems solved by technology

[0007] The purpose of the present invention is to provide an improved liquid chip kit for detecting acute coronary syndrome based on serum exosomes and its preparation method, which can overcome the related kits in the prior art in improving risk stratification of patients It is still a blank, and the specificity of related kits is not high.

Method used

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with embodiment.

[0041] The present invention mainly comprises that described liquid phase chip kit includes following components:

[0042] 1) Coated microspheres: Microspheres mainly include two types, one is polystyrene microspheres with a diameter of 5.6 μm, and the other is magnetic microspheres with a diameter of 6.5 μm. The above two types of microspheres are respectively coated with CD11c Capture antibody microspheres, CD81 capture antibody coated microspheres, MHCⅡ capture antibody coated microspheres, CD86 capture antibody coated microspheres, CD106 capture antibody coated microspheres, CD54 coated microspheres Microspheres capturing antibodies and microspheres coated with CD3 capturing antibodies, the above microspheres have different color codes respectively;

[0043] 2) Biotin-labeled detection antibodies: containing CD11c, CD81, MHCⅡ, CD86, CD106, CD54 and CD3 detection antibodies label...

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Abstract

The invention relates to a kit used for auxiliary judgment of risk stratification of patients with acute coronary syndrome based on detection of serum exosomes and a preparation method. The kit mainly comprises exosomes extraction reagents, relevant microballoons capturing antibodies (the above antibodies are one or some selected from CD11c, CD81, MHCII, CD86, CD106, CD54 and CD3), biotin labeled detection antibodies and streptavidin phycoerythrin. After being optimized, the diagnostic kit has advantages of high detection efficiency, less amount of required samples, high specificity, high sensitivity, fast and accurate detection and the like, so the risk stratification conditions of patients can be evaluated comprehensively, and relevant references are provided for clinical intervention.

Description

technical field [0001] The present invention relates to the technical field of in vitro kits, in particular to an in vitro kit for risk stratification of patients with acute coronary syndrome, in particular to a liquid phase chip combined parallel detection method based on serum exosomes, namely exosomes and Kit and preparation method thereof. Background technique [0002] Atherosclerosis (AS) vascular disease has become the "number one killer" that threatens the health of Chinese people. With the development of my country's economy, the incidence rate is increasing year by year. The annual medical expenses for such diseases in my country are as high as 100 billion Strengthening the prevention and treatment of AS vascular diseases and their complications has become the main task of the prevention and treatment of major diseases in our country. [0003] Acute coronary syndrome (ACS) caused by AS vascular disease is the most common and serious clinical manifestation, and the r...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 梁春贺治青王丽妍吴宗贵伍锋杨阳俞诚虹岳温恒
Owner 梁春
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