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Cadmium pollution bioremediation agent as well as preparation method and application thereof

A bioremediation agent and cadmium-polluted technology, which is applied in the field of agricultural microbiology, can solve problems such as safety standards and difficulty in medium and light pollution levels, and achieve the effects of reasonable types, reduced absorption, and strong comprehensive functions

Active Publication Date: 2013-10-23
HUNAN TAIGU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports on the application of microorganisms to remediate soil heavy metal pollution in China, and some scholars believe that it is difficult for bioremediation to meet the safety standards for medium and light pollution levels in a short period of time

Method used

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  • Cadmium pollution bioremediation agent as well as preparation method and application thereof
  • Cadmium pollution bioremediation agent as well as preparation method and application thereof
  • Cadmium pollution bioremediation agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The composition of embodiment 1 cadmium pollution bioremediation agent

[0035] 15 parts of core bacteria;

[0036] Nutritional ingredients 15 servings;

[0037] Enzyme active substance 4 parts;

[0038] 66 copies of mycocin carrier.

[0039] The composition of the core bacteria

[0040] Rhodopseudomonas palustris 40.0×10 8 cfu / g;

[0041] Bacillus cereus 10.0×10 8 cfu / g;

[0042] Brewer's yeast 10.0×10 8 cfu / g;

[0043] Total effective bacteria content ≥60.0×10 8 cfu / g.

[0044] The above-mentioned core bacterin of composition is made by the following steps:

[0045] Preparation of mixed spore powder:

[0046] ① Slant culture: Inoculate the original strains of Rhodopseudomonas palustris, Bacillus cereus and Saccharomyces cerevisiae on the slant medium under aseptic conditions, and culture at 29±1°C for 40 hours;

[0047] ②Shaking table culture: inoculate the strains cultured in the above step ① into the liquid medium under sterile conditions, and culture i...

Embodiment 2

[0057] The composition of embodiment 2 cadmium pollution bioremediation agent

[0058] 20 parts of core bacteria;

[0059] Nutritional ingredients 20 servings;

[0060] Enzyme active substance 5 parts;

[0061] 55 copies of mycocin carrier.

[0062] The composition of the core bacteria

[0063] Rhodopseudomonas palustris 30.0×10 8 cfu / g;

[0064] Bacillus cereus 15.0×10 8 cfu / g;

[0065] Brewer's yeast 15.0×10 8 cfu / g;

[0066] Total effective bacteria content ≥60.0×10 8 cfu / g.

[0067] Preparation:

[0068] ① Slant culture: Inoculate the original strains of Rhodopseudomonas palustris, Bacillus cereus and Saccharomyces cerevisiae on the slant medium under sterile conditions, and culture at 29±1°C for 48 hours;

[0069] ②Shaking table culture: inoculate the strains cultured in the above step ① into the liquid medium under sterile conditions, and culture in a shaking table at 160r / min for 48 hours at pH 7.0 and temperature 30°C;

[0070] ③ Fermentation tank culture:...

Embodiment 3

[0073] The composition of embodiment 3 cadmium pollution bioremediation agent

[0074] 10 parts of core bacteria;

[0075] Nutritional ingredients 10 servings;

[0076] 6 parts of enzyme active substance;

[0077] 74 copies of mycocin carrier.

[0078] The composition of the core bacteria

[0079] Rhodopseudomonas palustris 50.0×10 8 cfu / g;

[0080] Bacillus cereus 5.0×10 8 cfu / g;

[0081] Brewer's yeast 5.0×10 8 cfu / g;

[0082] Total effective bacteria content ≥60.0×10 8 cfu / g.

[0083] The medium used in each step when the above-mentioned implementation prepares the mixed spore powder is the same as the medium used in the corresponding steps of Example 1, and the preparation method of the product is the same. The proportions are different.

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Abstract

The invention provides a cadmium pollution bioremediation agent which is composed of the following ingredients in parts by weight: 10-20 parts of core rhzomorph, 10-20 parts of a nutritional ingredient, 2-6 parts of an enzyme activity substance and 54-78 parts of an rhzomorph carrier. The invention further provides an application of the cadmium pollution bioremediation agent. The cadmium pollution bioremediation agent is reasonable in microorganism specie collocation and high in comprehensive function. Microorganisms in the core rhzomorph can be planted and grow in soil rapidly to form strong bacterial colonies, and heavy metal ions, namely, cadmium Cd<+1> can be directly absorbed to microbial cell walls or into cells to be cured and passivated. Besides, bacillus cereus and beer yeast in the composition have high absorption and passivation function on heavy metal ions, namely, cadmium Cd<+1>, chromium Cr<+1> and lead Pb<+1>, due to the high comprehensive function, the mobility of the heavy metal ions, namely, cadmium Cd<+1>, is reduced, and the purpose of reducing absorption of crops on the heavy metal ions is achieved.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms, and in particular relates to a bioremediation agent containing active bacteria, a preparation method and application thereof. Background technique [0002] With the development of industrialization and agricultural intensification economy, the heavy metal pollution of cultivated land in my country is becoming more and more serious. In the early 1990s, the area of ​​cultivated land polluted by cadmium in my country reached 1.3×104 / hm 2 , involving 25 regions in 11 provinces and cities. According to the special survey of heavy metal pollution in Hunan Province and the monitoring results of agricultural environmental quality in recent years, the polluted cultivated land area in Hunan Province has accounted for 23.7% of the total cultivated land area in the province, and about 27% of the farmland irrigation water and 25% of the farmland The atmosphere has been polluted to vary...

Claims

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Application Information

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IPC IPC(8): C09K17/42C09K101/00
Inventor 唐八生曹典军郭帅蔡浩侯亚卓周艳周利群
Owner HUNAN TAIGU BIOTECH
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