Cadmium pollution bioremediation agent as well as preparation method and application thereof
A bioremediation agent and cadmium-polluted technology, which is applied in the field of agricultural microbiology, can solve problems such as safety standards and difficulty in medium and light pollution levels, and achieve the effects of reasonable types, reduced absorption, and strong comprehensive functions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] The composition of embodiment 1 cadmium pollution bioremediation agent
[0035] 15 parts of core bacteria;
[0036] Nutritional ingredients 15 servings;
[0037] Enzyme active substance 4 parts;
[0038] 66 copies of mycocin carrier.
[0039] The composition of the core bacteria
[0040] Rhodopseudomonas palustris 40.0×10 8 cfu / g;
[0041] Bacillus cereus 10.0×10 8 cfu / g;
[0042] Brewer's yeast 10.0×10 8 cfu / g;
[0043] Total effective bacteria content ≥60.0×10 8 cfu / g.
[0044] The above-mentioned core bacterin of composition is made by the following steps:
[0045] Preparation of mixed spore powder:
[0046] ① Slant culture: Inoculate the original strains of Rhodopseudomonas palustris, Bacillus cereus and Saccharomyces cerevisiae on the slant medium under aseptic conditions, and culture at 29±1°C for 40 hours;
[0047] ②Shaking table culture: inoculate the strains cultured in the above step ① into the liquid medium under sterile conditions, and culture i...
Embodiment 2
[0057] The composition of embodiment 2 cadmium pollution bioremediation agent
[0058] 20 parts of core bacteria;
[0059] Nutritional ingredients 20 servings;
[0060] Enzyme active substance 5 parts;
[0061] 55 copies of mycocin carrier.
[0062] The composition of the core bacteria
[0063] Rhodopseudomonas palustris 30.0×10 8 cfu / g;
[0064] Bacillus cereus 15.0×10 8 cfu / g;
[0065] Brewer's yeast 15.0×10 8 cfu / g;
[0066] Total effective bacteria content ≥60.0×10 8 cfu / g.
[0067] Preparation:
[0068] ① Slant culture: Inoculate the original strains of Rhodopseudomonas palustris, Bacillus cereus and Saccharomyces cerevisiae on the slant medium under sterile conditions, and culture at 29±1°C for 48 hours;
[0069] ②Shaking table culture: inoculate the strains cultured in the above step ① into the liquid medium under sterile conditions, and culture in a shaking table at 160r / min for 48 hours at pH 7.0 and temperature 30°C;
[0070] ③ Fermentation tank culture:...
Embodiment 3
[0073] The composition of embodiment 3 cadmium pollution bioremediation agent
[0074] 10 parts of core bacteria;
[0075] Nutritional ingredients 10 servings;
[0076] 6 parts of enzyme active substance;
[0077] 74 copies of mycocin carrier.
[0078] The composition of the core bacteria
[0079] Rhodopseudomonas palustris 50.0×10 8 cfu / g;
[0080] Bacillus cereus 5.0×10 8 cfu / g;
[0081] Brewer's yeast 5.0×10 8 cfu / g;
[0082] Total effective bacteria content ≥60.0×10 8 cfu / g.
[0083] The medium used in each step when the above-mentioned implementation prepares the mixed spore powder is the same as the medium used in the corresponding steps of Example 1, and the preparation method of the product is the same. The proportions are different.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com