Ice-temperature storage method of transplanted kidney and organs

A preservation method and kidney technology, applied in the field of protection and preservation of kidney organs, to achieve the effect of delayed preservation

Inactive Publication Date: 2013-10-16
TIANJIN UNIV OF COMMERCE
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Every 1°C decrease in the temperature of the perfused organ protection solution will cause many problems

Method used

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Embodiment Construction

[0012] The principle of the present invention will be further described below through examples. It should be noted that this embodiment is illustrative rather than limiting, and the protection scope of the present invention is not limited by this embodiment.

[0013] Embodiment Taking 30kg animal pig kidney as the preservation object, the specific method comprises the following steps:

[0014] (1) Intratracheal ether anesthesia, a midline upper abdominal incision was made, and the left or right kidney was removed as the donor kidney, and the renal artery was intubated immediately after the donor kidney was removed;

[0015] (2) Use 0-1°C organ protection solution to perfuse under gravity pressure, the perfusion flow rate is 2.7ml / s, the average cooling rate is 6.33°C / min, and the temperature of the protection solution flowing out of the kidney vein is 3.0°C stop;

[0016] (3) After irrigation, put the kidneys into a sterilized film bag containing 300ml of organ protection so...

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Abstract

The invention discloses an ice-temperature storage method of transplanted kidney and organs. The method specifically comprises the following steps of: carrying out ether anesthesia in trachea; taking the upper abdomen median incision, cutting left or right kidney as the donor kidney; intubating into the renal artery immediately after cutting the donor kidney; filling 0-1 DEG C of organ protective liquor by virtue of pushing force of an injection pump, wherein the filling flow rate is 2.7 ml/s-2.8ml/s, and the average cooling velocity is 6.00 DEG C/minute-6.33 DEG C/minute; and stopping the filling when the temperature of the filled protective liquid flowing out of the renal veins is 1.5 DEG C-2.5 DEG C; placing the kidney in a sterilized film bag with 300ml of organ protective liquid for sealing after filling, and storing the kidney in a constant-temperature box of 0 +/-0.15 DEG C. The ice-temperature technology is adopted, so that the organ tissue is lowered in phase-transition temperature and not frozen, so that the delay storage of the transplanted organ is realized; and the ice-point limit temperature is adopted for realizing the delay storage of the transplanted organ and to recover the good function of the transplanted organ.

Description

technical field [0001] The invention belongs to the field of organ transplantation medicine and biological heat transfer, and specifically relates to a technical method suitable for protecting and preserving kidney organs at freezing temperatures. Background technique [0002] At present, China has become one of the main countries in the world for kidney organ transplantation, and organ preservation is one of the three major technical pillars of organ transplantation. Especially for organ donors in other places, the loss of organ function due to improper organ preservation methods or preservation time has become the biggest regret in the shortage of donor organs. Therefore, people also realize that the current preservation methods are Limitations in preservation time and quality. As we all know, cryogenic freezing is usually used for the preservation of biological tissues, but for transplanted organs, if they are preserved at freezing temperatures, the freezing inside and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 诸凯王雅博梁飞张雅敏史源
Owner TIANJIN UNIV OF COMMERCE
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