Micro-fluidic chip structure for flow cytometer, and preparation method of micro-fluidic chip

Abstract

The invention discloses a micro-fluidic chip structure for a flow cytometer, and a preparation method a micro-fluidic chip. The chip comprises a sample liquid inlet 1, a column array structure 6, a conical focusing structure 7, a micro-channel 8, a detection zone 4, a flow expanding channel 9 and a waste liquid outlet end 5, wherein the column array structure 6 can play a role of filtering agglomerate protein impurities and other large biological solids inside sample liquid; and the conical focusing structure 7 has a focusing effect which is similar to that of the traditional sheath liquid system, so that cell granules flow inside the micro-channel 8 one by one; and the micro-channel 8 restricts the cells through the channel, so that the cells pass through the detection zone 4 one by one. By adopting the chip structure disclosed by the invention, the focusing effect without sheath flow liquid can be achieved; and blockage is not generated, so as to reduce use of the sheath flow liquid; and meanwhile, the situation that the cell granules pass through the detection zone one by one is ensured. The processing method of the micro-fluidic chip is convenient; the used bonding method is a common bonding method, and is simple and convenient to operate; and the used material PDMS (polydimethylsiloxane) is easy to process and has good biocompatibility.

Description

technical field [0001] The invention relates to a structure and a preparation method of a microfluidic chip used for a flow cytometer. Background technique [0002] Flow cytometry is a technology that automatically analyzes and detects cell particles in a liquid flow one by one. Flow cytometer (FCM) is based on the basic principles of flow cytometry, and comprehensively utilizes computer technology, laser technology, mechanical mechanics, cytochemistry, cellular immunology and other technologies to realize cell detection and analysis functions. instrument. Among them, the sample focusing is the key module of the flow cytometer, and the whole focusing process is to realize the focusing effect on the sample liquid through the action of external force. However, the entire system is bulky, complex, and expensive, and the emergence of microfluidic chip technology is expected to miniaturize the focus of cells, thereby miniaturizing the overall system. [0003] At present, the m...

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Problems solved by technology

Patent Publication No. US: 2009 / 0042241A1 adopts a simple constriction-type sheathless flow cell particle focusing method. Although this method can achieve the recommended focus of cell particles, the channel used is a small channel, which is easy to Blockage of the flow channel due to large particles of impurities, eventually rendering the entire chip ineffective

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