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Human umbilical cord mesenchymal stem cell culture medium

A technology of stem cells and culture medium, applied in the field of human umbilical cord mesenchymal stem cell culture medium, can solve the problems of stem cell activity, short form retention time, low viability rate, etc. Effect

Inactive Publication Date: 2015-08-26
张潇潇 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to solve the problems of short life and low viability of the stem cells when culturing human umbilical cord mesenchymal stem cells in the existing culture medium, and provide a human umbilical cord mesenchymal stem cell culture medium that solves the above problems

Method used

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  • Human umbilical cord mesenchymal stem cell culture medium
  • Human umbilical cord mesenchymal stem cell culture medium
  • Human umbilical cord mesenchymal stem cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this example, the basal medium is mixed with the following three concentrations of cysteine-rich secreted acidic protein, and the three specific concentrations are shown in Table 1a:

[0022] Table 1a

[0023] Numbering

concentration

①

1mg / L

②

1×10 -3 mg / L

③

1×10 -6 mg / L

[0024] Wherein, the composition of basal medium is shown in Table 1b:

[0025] Table 1b

[0026]

[0027]

[0028] Inject 5×10 in each ML culture medium 4 The cells were cultured, wherein three different concentrations of medium in Table 1a were used as the experimental group, and the basal medium was used as the control group. The experimental group and the control group were tested, and the test results were shown in Table 1c:

[0029] Table 1c

[0030]

[0031] When the number of cells in the 1ML culture medium was the largest, the cells were detected, and it was found that: the cells in the experimental group were all long s...

Embodiment 2

[0034] The difference between this example and Example 1 is that the composition of the protein added to the basal medium is different, and its specific composition is shown in Table 2a:

[0035] Table 2a

[0036]

[0037] Inject 5×10 in each ML culture medium 4 The cells were cultured, wherein three different concentrations of medium in Table 1a were used as the experimental group, and the basal medium was used as the control group. The experimental group and the control group were tested, and the test results were shown in Table 2b:

[0038] Table 2b

[0039]

[0040] When the number of cells in the 1ML culture medium was the largest, the cells were detected, and it was found that the cells in the experimental group were all long spindle-shaped, the nuclei were round or oval, and the average activity rate reached 97.3%; while in the control group The cells were spindle-shaped and fan-shaped, and the shape of the cells changed, and the nucleus in the fan-shaped cells ...

Embodiment 3

[0043] The difference between this example and Example 1 lies in the composition and concentration of the basal medium and protein. The specific composition of the basal medium is shown in Table 3a, and the specific composition of the protein is shown in Table 3b.

[0044] Table 3a

[0045] Element

Content (mg / L)

Element

Content (mg / L)

calcium nitrate tetrahydrate

100

L-serine

30

potassium chloride

400

L-threonine

20

Anhydrous Magnesium Sulfate

48.84

L-tryptophan

5

Sodium chloride

6000

L-tyrosine

20

Disodium phosphate

300

L-valine

20

L-Arginine

200

D-glucose

2000

L-Asparagine

50

Glutathione (reduced form)

1

L-Aspartic Acid

20

HEPES

3000

L-cystine hydrochloride

65.15

Phenol red

5

L-glutamic acid

20

Vitamin H

0.2

L-Glutamine

300

Calcium D-pantothenate

...

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Abstract

The invention discloses a human umbilical cord mesenchymal stem cell culture medium, which consists of a basal culture medium, an alpha-actinin 1 isotype b, alpha actin, lumican and excretory acidic protein rich in cysteine, wherein the concentration of the alpha-actinin 1 isotype b is 0.0001-1000ng / mL; the concentration of the alpha actin is 0.0001-1000ng / mL; the concentration of the lumican is 0.0001-1000ng / mL; and the concentration of the excretory acidic protein rich in cysteine is 0.0001-1000ng / mL. The human umbilical cord mesenchymal stem cell culture medium has the advantages of capabilities of keeping activities and form of stem cells for a long time, effective keeping own differentiation capacities of stem cells, and ensuring feature inheritance of stem cells, and the like.

Description

technical field [0001] The present invention relates to a culture medium, in particular to a human umbilical cord mesenchymal stem cell culture medium. Background technique [0002] Umbilical cord mesenchymal stem cells (Mesnchymal Stem Cells, MSCs) refer to a kind of multifunctional stem cells existing in the umbilical cord tissue of newborns. Umbilical cord mesenchymal stem cells (MSCs) have high differentiation potential and can differentiate into many kinds of tissues. It has broad clinical application prospects in tissue engineering such as bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. [0003] A variety of stem cell culture media have appeared, but the defect of the current stem cell culture media is that they are all general stem cell culture media, that is, the current stem cell culture media products are not aimed at a specific stem cell variety, but are used for a variety of Stem cell culture. However, due to the differe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
Inventor 朱一帆张潇潇王艳曹艳萍
Owner 张潇潇
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