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Method for determining the action factor of microbial preparations in reducing protein content in tobacco leaves

A technology of microbial preparation and protein content, applied in the field of biochemistry, can solve the problems of not being able to explain the reduction of protein in tobacco leaves, and unable to find factors that reduce protein in tobacco leaves, etc.

Inactive Publication Date: 2014-10-29
CHONGQING CHINA TOBACCO IND CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these traditional methods cannot explain the mechanism of protein reduction in tobacco leaves after spraying microorganisms, and cannot find the factors that reduce protein in tobacco leaves

Method used

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  • Method for determining the action factor of microbial preparations in reducing protein content in tobacco leaves
  • Method for determining the action factor of microbial preparations in reducing protein content in tobacco leaves
  • Method for determining the action factor of microbial preparations in reducing protein content in tobacco leaves

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Embodiment Construction

[0061] The following will be described in conjunction with the accompanying drawings and a specific embodiment.

[0062] The bacteria separated and screened from tobacco field soil are cultivated in the culture medium, the bacteria liquid is taken and centrifuged, and the supernatant is filtered to obtain metabolites. Perform SDS-PAGE on metabolites ( figure 1 ) and cut out the tape for enzymatic hydrolysis, and performed LC-MS / MS analysis on the enzymatic tape.

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Abstract

The invention discloses a method for measuring effect factors of a microorganism preparation for reducing the protein content in tobaccos. The method comprises the following steps of: extracting metabolites of a microorganism, performing sodium dodecyl sulfonate (SDS)-preview and graphics editing (PAGE) or dimensional electrophoresis after-cut adhesive tape or digging enzymolysis, and performing liquid chromatography-mass spectrometry / mass spectrometry (LC-MS / MS) analysis on an enzymolysis adhesive tape or a glue point; by multiple comparison treatment, performing chip hybridization on the treated tobaccos, and measuring a gene up-regulation expression and a gene down-regulation expression which are related to plant defense response, endogenous hormone metabolism, cell cycle control and enzyme control. The method has the beneficial effects that metabolic components capable of degrading the proteins in the tobaccos in the microorganism preparation from the molecule level and the change of the gene level of the tobaccos applied with the preparation can be analyzed, so that a mechanism of the microorganism preparation for degrading the proteins in the tobaccos can be comprehensively and clearly defined; theoretical basis is laid for further application of the biological preparation.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to a method for detecting the action factors of microbial agents reducing the protein content in tobacco leaves. Background technique [0002] Tobacco leaf, as the main product of tobacco, is not only an important research object as a model plant, but also the main raw material for industry. In my country, the protein content of upper tobacco leaves is relatively high. Protein is an important chemical component of tobacco, and its content is not only very important to the growth process of tobacco, but also closely related to the quality of tobacco. Microorganisms play a very important role in the growth, conditioning, aging, processing and storage of tobacco leaves. In recent years, microorganisms have been widely used to improve the chemical components in tobacco leaves to improve the quality of tobacco leaves. Wang Changguo and others sprayed the microorganisms screened from the soil ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02C12Q1/68
Inventor 赵敏汪长国戴亚李宁寇明钰吴艳刘一兵曾代龙杨军雷金山贾玉红胡希杨振廖占和
Owner CHONGQING CHINA TOBACCO IND CO LTD
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