Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture method for improving yield and viscosity of bacterial polysaccharides

A technology of bacterial polysaccharide and cultivation method, applied in the field of cultivation for improving the yield and viscosity of bacterial polysaccharide, can solve problems such as safety concerns, large cell damage, inability to transform, etc., so as to improve polysaccharide yield and viscosity, avoid harm, increase yield and The effect of viscosity

Inactive Publication Date: 2013-09-18
ZHEJIANG UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since gellan gum and xanthan gum are used as food additives, if the production strains are genetically engineered strains, consumers will worry about their safety
Moreover, some genes related to the biosynthetic pathway of polysaccharides have not been elucidated, so they cannot be modified by genetic engineering.
There are also studies to treat the production strains with physical or chemical mutagens, but because such mutagens cause greater damage to the cells, the positive mutation rate of high-yield and high-viscosity strains obtained is low, and the screening efficiency is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Determination of the minimum inhibitory concentration of ampicillin to xanthan gum producing bacteria Xanthomonas campestris and gellan gum producing bacteria Sphingomonas

[0020] 1. Xanthomonas campestris ATCC 13951 (purchased from ATCC) and Sphingomonas elodea ATCC 31461 (purchased from ATCC) were inoculated in YM liquid medium and cultured at 30°C and 200rpm until logarithmic growth Expect;

[0021] 2. The culture solution was transferred to 96-well plates containing different concentrations of ampicillin (10-200 μg / ml) at a volume ratio of 1:1000, and cultured at 30°C for 24 hours;

[0022] 3. Use a microplate reader to measure the optical density value OD at 0h of the culture and 24h after the end of the culture 595 ;

[0023] 4. The minimum inhibitory concentration is the minimum drug concentration that completely inhibits bacterial growth in the small hole;

[0024] 5. It has been determined that the minimum inhibitory concentration of ampicilli...

Embodiment 2

[0025] Embodiment 2: Continuous subculture of Xanthomonas campestris and Sphingomonas ampicillin stress mutagenesis

[0026] 1. Inoculate Xanthomonas campestris ATCC 13951 and Sphingomonas elodes ATCC 31461 in YM liquid medium, culture at 30°C, 200rpm for 24 hours; composition of YM liquid medium: peptone 5g / L, malt extract powder 3g / L, glucose 10g / L, yeast powder 3g / L, solvent is water, pH7.0;

[0027] 2. The above-mentioned cultures were transferred to YM liquid medium containing 50 μg / ml ampicillin at 10% inoculum size, and cultured at 30°C and 200 rpm for 24 hours;

[0028] 3. Continue to transfer 10% of the inoculum to the YM liquid medium containing 50 μg / ml ampicillin, culture at 30°C and 200 rpm for 24 hours; repeat this procedure to make the cells in the YM liquid medium containing 50 μg / ml ampicillin Passage 5 times;

[0029] 4. After Xanthomonas campestris and Sphingomonas were cultured and passaged 5 times in YM liquid medium containing 50 μg / ml ampicillin, the c...

Embodiment 3

[0031] Example 3: Screening of mutant strains in the continuous subculture process of ampicillin stress mutagenesis

[0032] 1. Absorb Xanthomonas campestris and sheaths of 55 passages (150 μg / ml ampicillin concentration), 105 passages (250 μg / ml ampicillin concentration) and 155 passages (350 μg / ml ampicillin concentration) respectively. Aminomonas culture solution, applied in gradient dilution on YM solid plate containing corresponding ampicillin concentration, cultured at 30°C for 72h;

[0033]2. Randomly select 20 single colonies from three culture dishes with different concentrations of Xanthomonas campestris and Sphingomonas, and transfer them to solid medium containing YM (add 15g / L agar), cultured at 30°C for 72h and set aside.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a culture method for improving yield and viscosity of bacterial polysaccharides. By using the characteristic that penbritin can be simultaneously used as a mutagenic agent and bacterial cell wall peptidoglycan to synthesize stress factors, the penbritin is added to subsequent subculture processes of bacterial isolates for producing different polysaccharides; the adding amount of the penbritin is gradually increased along with an increase of passage number, so as to improve thrill on bacterial cells; and a series of high-producing strains with different viscosities and improvement degrees can be screened in the continuous passage process, so as to adapt to different application requirements. Therefore, the production cost is reduced.

Description

(1) Technical field [0001] The invention relates to a cultivation method for increasing the yield and viscosity of bacterial polysaccharides. (2) Background technology [0002] Bacterial polysaccharides are secreted by bacterial cells and are polymers connected by multiple monosaccharide units or derivatives through condensation and dehydration. Bacterial polysaccharides mainly exist in nature in three forms: adhered to the cell surface, secreted into the culture medium, or formed into cellular components. According to the different repeating units, polysaccharides can generally be divided into homopolysaccharides and heteropolysaccharides. The former contains only one monosaccharide, while the latter contains two or more monosaccharides. Bacterial polysaccharides are produced by bacterial fermentation. Therefore, compared with animal and plant polysaccharides, their production is less affected by factors such as seasons, regions, pests and diseases, climate, natural disast...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01C12R1/64
Inventor 李欧郑道琼鲁翠刘傲吴雪昌
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com