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Preparation method and application of thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod

A technology of thrombin and aptamer, which is applied in the field of preparation of thrombin aptamer functionalized electrospinning enhanced solid-phase microextraction rod, can solve the problems of low specificity and small extraction capacity, and achieve strong specificity, The effect of high sensitivity, ultra-trace analysis and accurate analysis

Inactive Publication Date: 2014-11-05
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Coating materials are the key to the development of solid-phase microextraction technology. The current coating materials still have problems such as low specificity and low extraction capacity in the separation and enrichment of trace targets in complex matrices, especially in biological samples. Separation and analysis of proteins, etc. still face many challenges

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  • Preparation method and application of thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod
  • Preparation method and application of thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod
  • Preparation method and application of thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod

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Embodiment

[0024] In order to further explain the technical means and effects of the present invention to achieve the intended purpose of the invention, the following combined with the accompanying drawings and preferred embodiments, the thrombin aptamer functionalized electrospinning enhanced solid-phase microstructure proposed according to the present invention The preparation method of the extraction stick and its application are detailed as follows (the purity of the chemical reagents used in this example are all chemical pure).

[0025] 1. Specific steps of the preparation method of thrombin aptamer functionalized electrospinning enhanced solid-phase microextraction rod:

[0026] (1) Polyacrylonitrile-maleic acid polymer was synthesized by radical aqueous precipitation polymerization: 10.6 grams of acrylonitrile and 7.4 grams of maleic acid were added to a round-bottomed flask, and then 20 milliliters of deionized water at 60 °C were added , constant temperature water bath and stirr...

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Abstract

The invention discloses preparation and application of a thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod. The thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod is prepared through the following steps of: synthesizing a polyacrylonitrile-maleic acid polymer by adopting a free radical water-phase precipitation polymerization method; then preparing a solid-phase extraction rod which has the advantages of large surface area, high stability and high durability by directly taking a stainless steel wire as a substrate and adopting an electrospining technology; and fixing a thrombin aptamer to the surface of the solid-phase extraction rod by adopting a carbodiimide method. The prepared thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod can be applied to the isolation analysis of a trace amount of thrombin contained in complex samples, such as human serum, human plasma, human blood, and the like. The method disclosed by the invention can be used for preparing a solid-phase microextraction base body coating and controlling the thickness of the solid-phase microextraction base body coating by taking the stainless steel wire as the substrate and adopting an electrospining technology; the solid-phase microextraction base body coating is uniform, loose, porous and large in surface area; and the prepared solid-phase microextraction rod has the advantages of high specificity and high stability.

Description

technical field [0001] The invention belongs to the technical field of analytical chemical sample pretreatment, and particularly relates to a preparation method of a thrombin aptamer functionalized electrospinning enhanced solid phase microextraction rod. The prepared thrombin aptamer functionalized electrospinning enhanced solid Phase microextraction rods are suitable for the separation, purification and analysis of traces of target components in complex matrices in the fields of environment, food, medicine and biology. Background technique [0002] Thrombin is a proteolytic enzyme formed from the precursor of thrombin (an essential component in plasma), which catalyzes the conversion of fibrinogen into fibrin to promote blood coagulation. The amount of thrombin can be used for the diagnosis of some diseases, such as the monitoring of hereditary bleeding disorders or coagulation disorders; on the other hand, thrombin is used to ligate small blood vessels, capillaries and so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N9/74G01N1/28C08F220/44C08F222/06D01F6/40D01D5/00
Inventor 杜甫佑孙林阮贵华
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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