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Agricultural phytosterol bactericide, and preparation method and application thereof

A technology of agricultural fungicides and phytosterols, applied in the field of pesticides, can solve the problems that there are no reports on the application of phytosterols, and achieve a promising effect in the market

Inactive Publication Date: 2013-08-28
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phytosterols have a variety of physiological functions. They are widely used in medicine, cosmetics, animal growth agents, paper processing, printing, textiles, food, etc., but there are no reports on the application of phytosterols in the preparation of agricultural fungicides.

Method used

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  • Agricultural phytosterol bactericide, and preparation method and application thereof
  • Agricultural phytosterol bactericide, and preparation method and application thereof
  • Agricultural phytosterol bactericide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the extraction of phytosterol

[0026] (1) Collect vitex flowers, soak and extract with appropriate amount of 80% ethanol for 3 times, combine the filtrates from 3 times, concentrate under reduced pressure, freeze-dry, and set aside. The ethanol extract of Vitex twigs is made into a suspension with appropriate amount of distilled water, extracted with petroleum ether, ethyl acetate and n-butanol respectively, the obtained extract and the remaining water phase are concentrated under reduced pressure, freeze-dried, and set aside.

[0027] (2) The active components in the petroleum ether extract were separated by silica gel column chromatography. Use 1500g of 200-300 mesh silica gel powder, use wet method to pack the column, dissolve 72.3g of petroleum ether phase extract with an appropriate amount of petroleum ether, add 100g of 200-300 mesh silica gel powder to mix the sample, add the sample to the top of the column, and successively use petroleum Ether, p...

Embodiment 2、5

[0028] The preparation of embodiment 2,5% stigmasterol wettable powder

[0029] (1) the extraction of sterol, with embodiment 1

[0030] (3) 40% (weight percent) of stigmasterol, 5% (weight percent) of sodium lauryl sulfate, and 55% (weight percent) of diatomaceous earth, which are mixed evenly and made into a wettable powder through a jet mill.

[0031] (4) The sterol preparation was made into five concentration gradients of 0.4, 0.2, 0.1, 0.05, and 0.025 mg / mL, and the antibacterial activities of apple rot fungus, cabbage black spot fungus and grape white rot fungus were determined by growth rate method.

Embodiment 3

[0032] The preparation of embodiment 3.3% stigmasterol wettable powder

[0033] Except step (3) sterol substance is 3% (percentage by weight), all the other are identical with embodiment 2.

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PUM

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Abstract

The invention belongs to the pesticide field, and concretely relates to an agricultural phytosterol bactericide. The bactericide contains 3-70% of an effective active component. Phytosterol can be added into a wetting agent, a dispersant, a filler and the like to prepare a wettable pulvis, can be added into antifreeze agent, a wetting dispersant, a thickening agent, an antiseptic and water in proportion to prepare a suspending agent, can also be added into an emulsifier, a solvent and water in proportion to prepare an emulsion in water. The agricultural phytosterol bactericide can be used for controlling crop, fruit and vegetable diseases comprising Cytospora sp, Alternaria oleracea, Coniella diplodiella and the like. According to the invention, phytosterol is from plants, is well compatible to the environment and is easy to degrade, so the phytosterol bactericide is a botanical bactericide and has a wide market prospect.

Description

technical field [0001] The invention belongs to the field of pesticides, in particular to an agricultural fungicide. Background technique [0002] Spraying chemical pesticides to prevent and control plant diseases and insect pests in the process of agricultural production management and storage is still an important means of plant protection. Its advantages are quick effect, convenient operation, low cost, and less restricted by environmental factors. However, relying solely on and using chemical pesticides for a long time will cause aggravated changes in the pest flora, and dangerous pests will continue to occur and become more and more serious. The "three R's" problem is becoming more and more prominent. With the rapid development of the economy and the continuous improvement of people's living standards, food safety has become the focus of people's attention and has developed into a worldwide problem. People pay more and more attention to the threat of pesticide residue...

Claims

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Application Information

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IPC IPC(8): A01N45/00A01N43/22A01P3/00
Inventor 罗兰袁忠林
Owner QINGDAO AGRI UNIV
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