Application of punicalin in preparation of medicine for treating or preventing hepatitis C virus infection

A technology for hepatitis C virus and pomegranate tannin, which is applied in the field of medicine, can solve the problem of no application of pomegranate tannin in anti-hepatitis C virus and the like, and achieve the effect of inhibiting entry and replication.

Inactive Publication Date: 2013-08-14
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no application of pomegranate tannin against HCV

Method used

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  • Application of punicalin in preparation of medicine for treating or preventing hepatitis C virus infection
  • Application of punicalin in preparation of medicine for treating or preventing hepatitis C virus infection
  • Application of punicalin in preparation of medicine for treating or preventing hepatitis C virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Evaluation of anti-hepatitis C virus activity of pomegranate skin tannins

[0034] 1. Experimental materials

[0035] 1.1 Cells, plasmids, viruses and drugs

[0036] Huh7.5.1 cells were donated by Dr.F.V.Chisari; the plasmid pJFH1 containing the complete genome sequence of HCV2a type JFH1 strain was donated by Prof. Dr. Takaji Wakita. The virus JFH1-5AGFP with the reporter gene was prepared by the applicant's laboratory (refer to step B "Evaluation of anti-hepatitis C virus activity of pomegranate tannins" or refer to Wu Y, Liao Q, Yang R, et al.A novel luciferase and GFP dual reporter virus for rapid and convenient evaluation of hepatitis C virus replication[J].Virus Research,2011,155(2):406-414.); pomegranate peel tannin was purchased from Shanghai Kaiyang Biotechnology Co., Ltd.

[0037] 1.2 Reagents

[0038] DMEM medium was purchased from GIBCO Company; MTT detection reagent was purchased from biomol Company; restriction endonuclease was purchased f...

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PUM

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Abstract

The invention discloses application of punicalin in preparation of medicine for treating or preventing hepatitis C virus infection. An antivirus test is performed by selecting basically non-toxic medicine concentration, the influence of punicalin on copy of hepatitis C virus is detected, and the compound has obvious hepatitis C virus resistant activity and has a dose-dependent effect. The further experiment proves that the punicalin can prevent HCV from being adsorbed and invading the target cells, so that the entrance and reinfection of HCV are inhibited; and meanwhile, the compound also can obviously reduce the activity of HCVNS3 serine protease. Thus, when being independently used or combined with other anti-HCV medicines, the punicalin can play an important role in the process of preventing or treating hepatitis C virus infection.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to the application of pomegranate tannin in the preparation of medicines for treating or preventing hepatitis C virus (HCV) infection. Background technique [0002] Hepatitis C virus (Hepatitis C Virus, HCV) was discovered in 1989, it is the main pathogen causing non-A non-B hepatitis after blood transfusion. About 170 million people worldwide are currently infected with HCV. HCV infection is distributed worldwide, with an infection rate of about 3%, of which 3 to 4 million new cases are added each year. According to the serological epidemiological survey data in my country, the positive rate of HCV in the general population is 3.2%. The main routes of transmission of HCV include blood transfusion or blood products, intravenous drug use, sexual contact, and mother-to-child transmission. HCV infection can easily lead to chronicity, and only about 20% of HCV-infect...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/14
Inventor 陈绪林郭文炎吴阳廖庆姣
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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