Method for carrying out tissue culture and seedling growing of michelia champaca

A technology for the group and tissue culture seedlings of Amur japonica is applied in the field of cultivating seedlings of arbor garden plant groups, which can solve the problems of low natural reproduction rate, inability to meet market demands, limited reproduction number, etc., and achieves reduction of browning death and elimination of browning death. , good value-added effect

Active Publication Date: 2015-03-18
YIBIN YUNCHEN ARBOR GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the degeneration of pistils and stamens, generally all can not produce fruits and seeds, its natural reproduction rate is extremely low, and its seeds are few and easily inactivated.
The stem cell differentiation degree of Jujube orchid is high, the ability of reproduction and regeneration is poor, and the cuttings are not easy to take root. People's research on the tissue culture technology of Jujube orchid is still in the exploratory stage
[0003] At present, the methods of grafting and high-altitude layering are mainly adopted commercially for reproduction. This kind of method takes a long time to reproduce and the number of reproduction is extremely limited, which is far from meeting the market demand.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for cultivating seedlings of the Huangjuan group includes the following steps:

[0024] 1. Selection and disinfection of explants:

[0025] Choose yellow juniper twigs that grow sturdy, have no diseases and insect pests, and are semi-lignified and with 3-5 full side buds and top buds. Go back to the laboratory and cut off the leaves, wipe the surface of the branches with a cotton ball dipped in 0.2% neoceramic, and rinse with running water for 30 minutes, while gently scrubbing the dust on the explants. Then cut the branches into small sections with 1-2 buds, soak them in 70% alcohol for 10 seconds, rinse once with sterile water, soak the material with 0.1% mercuric chloride for 5 minutes, rinse 4 times with sterile water, and rinse with 5 Soaked in% sodium hypochlorite solution for 5 minutes, and finally rinsed with sterile water for 5 times, each rinse with sterile water for 30 seconds, use sterile paper to absorb residual moisture on the material for later use. ...

Embodiment 2

[0035] A method for cultivating seedlings of the Huangjuan group includes the following steps:

[0036] 1. Selection and disinfection of explants:

[0037] Choose yellow juniper twigs that grow sturdy, have no diseases and insect pests, and are semi-lignified and with 3-5 full side buds and top buds. Go back to the laboratory and cut off the leaves, wipe the surface of the branches with a cotton ball dipped in 0.3% neocermate, and then rinse with running water for 50 minutes, during which time the dust on the explants is gently scrubbed. Then cut the branches into small segments with 1-2 buds, soak in 70% alcohol for 12 seconds, rinse once with sterile water, soak the material with 0.5% mercuric chloride for 6 minutes, rinse 4 times with sterile water, and use 8% Soak in the sodium hypochlorite solution for 5 minutes, and finally rinse with sterile water for 5 times. Each sterile water rinsing time is 50 seconds. Use sterile paper to absorb the residual water on the material for l...

Embodiment 3

[0047] A method for cultivating seedlings of the Huangjuan group includes the following steps:

[0048] 1. Selection and disinfection of explants:

[0049] Choose yellow juniper twigs that grow sturdy, have no diseases and insect pests, and are semi-lignified and with 3-5 full side buds and top buds. Go back to the laboratory and cut off the leaves, wipe the surface of the branches with a cotton ball dipped in 0.5% neoceramic, and then rinse with running water for 60 minutes, while gently scrubbing the dust on the explants. Then cut the branches into small sections with 1-2 buds, soak them in 70% alcohol for 15 seconds, rinse once with sterile water, soak the material with 1.0% mercuric chloride for 8 minutes, rinse 4 times with sterile water, Soaked in% sodium hypochlorite solution for 5 minutes, and finally rinsed with sterile water 5 times, each rinse time with sterile water is 60 seconds, use sterile paper to absorb residual water on the material for later use.

[0050] 2. Inoc...

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PUM

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Abstract

The invention provides a method for carrying out tissue culture and seedling growing of michelia champaca. The method comprises the following steps of: selecting annual semi-lignified michelia champaca twigs which grow robustly, have no plant diseases and insect pests, and have 3-5 full lateral buds and terminal buds; cutting off leaves; disinfecting and sterilizing surfaces; cutting the twigs into small sections with 1-2 buds; and carrying out pre-culture, primary culture, secondary culture, rooting culture and tissue culture seedling domestication, and transplanting into a greenhouse or a flower pot to be cultured. With the adoption of the method disclosed by the invention, the pollution to explants in a primary culture phase is controlled and reduced; a phenomenon that the explants easily have browning and die during a primary culture period is reduced and eliminated; the success rate of the tissue culture of the michelia champaca is improved; the good increment effect of the tissue culture of the michelia champaca and a lot of tissue-cultured, bred and grown seedlings can be realized; and the difficulties that the bred and grown seedlings of the michelia champaca cannot easily grow roots to survive, time for grafting and layering rooting is long, the breeding quantity is less and the requirements on michelia champaca nursery-grown plants by a market are difficult to meet, which trouble people for a long time, can be solved.

Description

Technical field [0001] The invention relates to a method for cultivating seedlings of a tree garden plant group, in particular to a method for cultivating seedlings of the yellow pineapple group. Background technique [0002] Yellow juniper (scientific name: Michelia champaca) is an evergreen broad-leaved tree of the genus Magnoliaceae, also known as white orchid and yellow magnolia. Yellow pineapple is a famous ornamental greening plant and a precious floral tree species. The flowers and leaves are uniform, the branches are straight, the tree shape is beautiful, the leaves are green, the flowering period is long, the flowers are white and elegant, the flowers are rich in fragrance, and the fragrance is fresh and elegant. Yellow pineapple shows strong resistance and absorption ability to harmful gases such as sulfur dioxide and chlorine, and is a good anti-pollution greening tree species. At the same time, yellow pineapple, as a spice plant, can also be used as a spice and medici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 何素芬顾大勤
Owner YIBIN YUNCHEN ARBOR GARDEN
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