Method for carrying out tissue culture and seedling growing of michelia champaca
A technology for the group and tissue culture seedlings of Amur japonica is applied in the field of cultivating seedlings of arbor garden plant groups, which can solve the problems of low natural reproduction rate, inability to meet market demands, limited reproduction number, etc., and achieves reduction of browning death and elimination of browning death. , good value-added effect
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Embodiment 1
[0023] A method for cultivating seedlings of the Huangjuan group includes the following steps:
[0024] 1. Selection and disinfection of explants:
[0025] Choose yellow juniper twigs that grow sturdy, have no diseases and insect pests, and are semi-lignified and with 3-5 full side buds and top buds. Go back to the laboratory and cut off the leaves, wipe the surface of the branches with a cotton ball dipped in 0.2% neoceramic, and rinse with running water for 30 minutes, while gently scrubbing the dust on the explants. Then cut the branches into small sections with 1-2 buds, soak them in 70% alcohol for 10 seconds, rinse once with sterile water, soak the material with 0.1% mercuric chloride for 5 minutes, rinse 4 times with sterile water, and rinse with 5 Soaked in% sodium hypochlorite solution for 5 minutes, and finally rinsed with sterile water for 5 times, each rinse with sterile water for 30 seconds, use sterile paper to absorb residual moisture on the material for later use. ...
Embodiment 2
[0035] A method for cultivating seedlings of the Huangjuan group includes the following steps:
[0036] 1. Selection and disinfection of explants:
[0037] Choose yellow juniper twigs that grow sturdy, have no diseases and insect pests, and are semi-lignified and with 3-5 full side buds and top buds. Go back to the laboratory and cut off the leaves, wipe the surface of the branches with a cotton ball dipped in 0.3% neocermate, and then rinse with running water for 50 minutes, during which time the dust on the explants is gently scrubbed. Then cut the branches into small segments with 1-2 buds, soak in 70% alcohol for 12 seconds, rinse once with sterile water, soak the material with 0.5% mercuric chloride for 6 minutes, rinse 4 times with sterile water, and use 8% Soak in the sodium hypochlorite solution for 5 minutes, and finally rinse with sterile water for 5 times. Each sterile water rinsing time is 50 seconds. Use sterile paper to absorb the residual water on the material for l...
Embodiment 3
[0047] A method for cultivating seedlings of the Huangjuan group includes the following steps:
[0048] 1. Selection and disinfection of explants:
[0049] Choose yellow juniper twigs that grow sturdy, have no diseases and insect pests, and are semi-lignified and with 3-5 full side buds and top buds. Go back to the laboratory and cut off the leaves, wipe the surface of the branches with a cotton ball dipped in 0.5% neoceramic, and then rinse with running water for 60 minutes, while gently scrubbing the dust on the explants. Then cut the branches into small sections with 1-2 buds, soak them in 70% alcohol for 15 seconds, rinse once with sterile water, soak the material with 1.0% mercuric chloride for 8 minutes, rinse 4 times with sterile water, Soaked in% sodium hypochlorite solution for 5 minutes, and finally rinsed with sterile water 5 times, each rinse time with sterile water is 60 seconds, use sterile paper to absorb residual water on the material for later use.
[0050] 2. Inoc...
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