Method for carrying out tissue culture and seedling growing of michelia champaca

Abstract

The invention provides a method for carrying out tissue culture and seedling growing of michelia champaca. The method comprises the following steps of: selecting annual semi-lignified michelia champaca twigs which grow robustly, have no plant diseases and insect pests, and have 3-5 full lateral buds and terminal buds; cutting off leaves; disinfecting and sterilizing surfaces; cutting the twigs into small sections with 1-2 buds; and carrying out pre-culture, primary culture, secondary culture, rooting culture and tissue culture seedling domestication, and transplanting into a greenhouse or a flower pot to be cultured. With the adoption of the method disclosed by the invention, the pollution to explants in a primary culture phase is controlled and reduced; a phenomenon that the explants easily have browning and die during a primary culture period is reduced and eliminated; the success rate of the tissue culture of the michelia champaca is improved; the good increment effect of the tissue culture of the michelia champaca and a lot of tissue-cultured, bred and grown seedlings can be realized; and the difficulties that the bred and grown seedlings of the michelia champaca cannot easily grow roots to survive, time for grafting and layering rooting is long, the breeding quantity is less and the requirements on michelia champaca nursery-grown plants by a market are difficult to meet, which trouble people for a long time, can be solved.

Description

Technical field [0001] The invention relates to a method for cultivating seedlings of a tree garden plant group, in particular to a method for cultivating seedlings of the yellow pineapple group. Background technique [0002] Yellow juniper (scientific name: Michelia champaca) is an evergreen broad-leaved tree of the genus Magnoliaceae, also known as white orchid and yellow magnolia. Yellow pineapple is a famous ornamental greening plant and a precious floral tree species. The flowers and leaves are uniform, the branches are straight, the tree shape is beautiful, the leaves are green, the flowering period is long, the flowers are white and elegant, the flowers are rich in fragrance, and the fragrance is fresh and elegant. Yellow pineapple shows strong resistance and absorption ability to harmful gases such as sulfur dioxide and chlorine, and is a good anti-pollution greening tree species. At the same time, yellow pineapple, as a spice plant, can also be used as a spice and medici...

AI Technical Summary

Problems solved by technology

However, due to the degeneration of pistils and stamens, generally all can not produce fruits and seeds, its natural reproduction rate is extremely low, and its seeds are few and easily inactivated.

The stem cell differentiation degree of Jujube orchid is high, the ability of reproduction and regeneration is poor, and the cuttings are not easy to take root. People's research on the tissue culture technology of Jujube orchid is still in the exploratory stage

[0003] At present, the methods of grafting and high-altitude layering are mainly adopted commercially for reproduction. This kind of method takes a long time to reproduce and the number of reproduction is extremely limited, which is far from meeting the market demand.