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A kind of snail extract containing 6-bromoindolindione and its preparation method and its anticancer application

A technology of litchi snails and extracts, applied in the field of medicine, can solve the problem of low anti-tumor effect, achieve good inhibitory effect, obvious apoptosis of liver cancer cells, and low cost

Inactive Publication Date: 2015-11-18
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are few domestic studies on the chemical components and anti-tumor effects of snails, especially the effects on liver cancer cells have not been reported

Method used

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  • A kind of snail extract containing 6-bromoindolindione and its preparation method and its anticancer application
  • A kind of snail extract containing 6-bromoindolindione and its preparation method and its anticancer application
  • A kind of snail extract containing 6-bromoindolindione and its preparation method and its anticancer application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the preparation of the snail extract containing 6-bromoindolindione

[0054] 1. Preparation of snail extract

[0055] as follows figure 1 Separate the snail mollusk or strip the glandular tissue as indicated, and follow the procedure described ( figure 2 ) separation and extraction to obtain a brownish-yellow powder which is the snail extract.

[0056] method one:

[0057] ①Crack the snail shell (5kg), take the soft part, and use tweezers to separate the subparotid gland, digestive gland and rectal gland tissue ( figure 1 ), freeze-dried in vacuum for 48 hours, obtained 20 g of dry tissue and ground it into powder, and obtained dried Snail gland powder.

[0058] ②Take the dried snail gland powder (20g) and place it in a brown bottle (protected from light), and use 1L of 80% (volume percentage) ethanol aqueous solution to stir the 20g snail gland powder at room temperature 25°C with magnetic force. Extract in 3 times (the total amount of ethanol aqueo...

Embodiment 2

[0067] Embodiment 2, the application of the snail extract containing 6-bromoindolindione in anticancer

[0068] 1. MTT method to detect the inhibitory effect of snail extract containing 6-bromoindolindione on the proliferation of liver cancer QGY-7703 and SMMC-7721 cells

[0069] (1) Liver cancer QGY-7703 (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences, catalog number TCHu43) and SMMC-7721 (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences, catalog number TCHu52) were inoculated respectively in RPMI-1640 medium containing 10% fetal bovine serum at 37°C, 5% CO 2 , CO at saturated humidity 2 Routinely cultured in an incubator (Thermo371, USA), the medium was changed every 2 days, and the cells in the logarithmic growth phase were used for experiments.

[0070] (2) Inoculate various logarithmic growth phase cells obtained in the above (1) in a 96-well cell culture pl...

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Abstract

The invention discloses a dog whelk extract containing 6-bromo-1H-indole-2, 3-dione, a preparation method thereof and an anti-caner application thereof. The dog whelk extract preparation method provided by the invention comprises the following steps: 1, soaking dog whelk soft tissues into an alcohol aequeous solution, collecting an extracting solution, and concentrating at reduced pressure so as to obtain the extract, wherein the dog whelk soft tissues are cheek gland, digestive gland and rectal gland tissues; and 2, dissolving the extract into the water so as to obtain an extract aqueous solution; and utilizing normal hexane or ethyl acetate to extract the extract aqueous solution, collecting organic phases, and carrying out silica column chromatographic isolation so as to obtain the dog whelk extract containing 6-bromo-1H-indole-2, 3-dione. Experiments prove that the obtained dog whelk extract containing 6-bromo-1H-indole-2, 3-dione has a good inhibiting effect on hepatoma carcinoma cell proliferation; and search results on a function mechanism of the dog whelk extract containing 6-bromo-1H-indole-2, 3-dione discloses that the dog whelk extract containing 6-bromo-1H-indole-2, 3-dione has an obvious induction function on hepatoma carcinoma cell apoptosis.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a snail extract containing 6-bromoindolindione, its preparation method and its anticancer application. Background technique [0002] Due to the high prevalence of hepatitis B in my country, the incidence of primary liver cancer remains high, accounting for about 55% of the total number of liver cancer patients in the world. It is one of the major diseases that seriously threaten the health of the people in our country. Drugs and treatments are imminent. The ocean occupies 2 / 3 of the earth's surface area, and there are as many as 210,000 known species of microorganisms, lower or higher animals and plants inhabiting the ocean water body. Due to the particularity and biodiversity of the ocean environment, the metabolic enzyme system of marine organisms and Terrestrial organisms are quite different, and secondary metabolites derived from marine organisms have unique physiological activities a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/38A61P35/00
Inventor 林祥志张芳马伏宁张林马瑞娟荣辉林汝榕王昭凯杨善军马勇陈水波柯秀蓉李惠丽
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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