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Preparation method of oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed

A technology for steelhead trout and triploid, which is applied in the field of preparation of hybrid triploid seedlings of golden trout and steelhead trout, can solve the problem of chemical sensitivity, high water quality requirements for growth environment, and lack of 6-dimethyl Aminopurines and other issues

Inactive Publication Date: 2014-12-10
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 6-Dimethylaminopurine (N6-Dimethyladenine, 6-DMAP) has achieved good results in the induction of polyploidy in various shellfish, but because salmon trout have high requirements on the water quality of the growth environment and are particularly sensitive to chemical substances, So far, there is no successful report on the application of 6-dimethylaminopurine in the induction of triploid or hybrid triploid in salmon and trout

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Method for preparing hybrid triploid seed of golden trout and steelhead trout (1)

[0028] By means of water stimulation, light control, and enhanced compound feed for broodstock, broodstock of golden trout and broodstock of steelhead trout can develop and mature synchronously. The water temperature for broodstock breeding of golden trout and steelhead trout is 10°C, and the water exchange volume in the running pool is 2h / time.

[0029] The eggs are fertilized by dry method. Squeeze the roe of mature female golden trout into a clean porcelain basin first, and then squeeze the semen of 2 male steelhead trout into a water basin with water temperature of 8 ℃, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:2500, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0030] After 12 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-...

Embodiment 2

[0032] Example 2 Method for preparing hybrid triploid seed of golden trout and steelhead trout (2)

[0033] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make golden trout and steelhead trout broodstock develop and mature synchronously. The water temperature for broodstock breeding of golden trout and steelhead trout is 13°C, and the water exchange volume in the running pool is 2h / time.

[0034] The eggs are fertilized by dry method. Squeeze the roe of mature female golden trout into a clean porcelain basin first, and then squeeze the semen of 2 male steelhead trout into a water basin with water temperature of 8 ℃, stir immediately, add hatching water with a volume ratio of semen to hatching water of 1:3000, stir gently, pour out the water after 1.5 minutes, repeat 3 times, clean the semen, and place it for processing.

[0035] After 13 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solu...

Embodiment 3

[0037] Example 3 Method for preparing hybrid triploid seed of golden trout and steelhead trout (3)

[0038] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make golden trout and steelhead trout broodstock develop and mature synchronously. The water temperature for broodstock breeding of golden trout and steelhead trout is 11°C, and the water exchange volume in the running pool is 2h / time.

[0039]The eggs are fertilized by dry method. Squeeze the roe of mature female golden trout into a clean porcelain basin first, and then squeeze the semen of 2 male steelhead trout into a water basin with water temperature of 8 ℃, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1.5:3000, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0040] After 14 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP so...

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Abstract

The invention provides a preparation method of an oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed. The preparation method comprises the steps of: carrying out in vitro fertilization on oncorhynchus mykiss walbaum female parent fish ova and oncorhynchus mykiss male parent fish sperms, transferring into a 170-190 mg / L 6-DMAP (6-dimethylaminopyridine) solution with the temperature of 12-14 DEG C after fertilized ova is fertilized for 12-14minutes, soaking for 14-16minutes, then transferring in a water body with the temperature of 6-8 DEG C and then hatching; hatching the fertilized ovum to obtain hybrid triploid larva fishes after about 50 days; and detecting the larva fishes by using a flow cytometry, wherein the eyed rate of the fertilized ova in a hatching period is above 71 percent, the hatching rate of the seeds reaches above 90 percent and the triploid rate reaches above 80 percent. By using the preparation method of the oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed, the problems of sexual precocity, miniaturization, disease propagation and the like of individuals in a trout cultivating process are solved, the advantages of hybridization and triploid are obtained, and a good market value is achieved.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a method for preparing hybrid triploid seedlings of golden trout and steelhead trout. Background technique [0002] Salmon trout is one of the main farmed fish in the world at present. Due to its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon and trout are currently the main cold-water cultured fish in my country. With the rapid development of salmon and trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon and trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞杨贵强
Owner BEIJING FISHERIES RES INST
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