Method for preparing collagen protein powder from fish scales
A technology of collagen protein powder and fish scales, which is applied in the direction of protein composition of fish, protein food composition, food science, etc., to achieve the effects of low production cost, shortened production cycle, and improved comprehensive utilization rate
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Embodiment 1
[0027] 1) Deodorization and decalcification of fish scales
[0028] Put the fish scales collected in the process of silver carp processing into the container, by weight, add tap water to the fish scales by fish scales: water=1:1, then add the Bacillus subtilis bacterium liquid of 3% of the above-mentioned material gross weight, at 35 ℃, 180r / min shaking culture for 2 hours for deodorization treatment, then add 2% by weight of HCl solution with a mass concentration of 5% to the obtained material, and shake and react at 150r / min for 10 minutes for decalcification treatment; The fish scales are washed repeatedly with clean water to remove the silt, bacteria and impurities, and cleaned for later use;
[0029] Wherein the Bacillus subtilis bacteria liquid is obtained by expanding the slant strain of the Bacillus subtilis in a shake flask, and the culture conditions are: 39° C., 120 r / min shaking culture for 24 hours.
[0030] 2) Frozen crushing treatment
[0031] Freeze the washe...
Embodiment 2
[0040] 1) Deodorization and decalcification of fish scales
[0041] Put the fish scales collected during grass carp processing into a container, and add tap water to the fish scales according to fish scales: water = 1:1 by weight, and then add 5% of the total weight of the above-mentioned materials. , 120r / min shaking and culturing for 1 hour for deodorization treatment, then adding 3% by weight of HCl solution with a mass concentration of 5% to the obtained material, and carrying out decalcification treatment at 180r / min shaking reaction for 12 minutes; fish scales after treatment Rinse with clean water repeatedly to remove the sediment, bacteria and impurities, and clean it for later use;
[0042] Wherein, the Bacillus subtilis bacteria liquid is obtained by expanding the culture of the slant strain of Bacillus subtilis in a shake flask, and the culture conditions are: 35°C, 150r / min shaking culture for 30 hours;
[0043] 2) Frozen crushing treatment
[0044] Freeze the wa...
Embodiment 3
[0053] 1) Deodorization and decalcification of fish scales
[0054] Put the fish scales collected during the herring processing into a container, add tap water to the fish scales according to fish scales: water = 1:1 by weight, and then add 4% of the total weight of the above-mentioned materials. , 150r / min shaking and culturing for 1.5 hours for deodorization treatment, then adding 2% by weight of HCl solution with a mass concentration of 5% to the obtained material, and performing decalcification treatment at 150r / min shaking reaction for 10 minutes; the treated fish scales Rinse with clean water repeatedly to remove the sediment, bacteria and impurities, and clean it for later use;
[0055] Wherein the Bacillus subtilis bacteria liquid is obtained by expanding the slant strain of Bacillus subtilis in a shake flask, and the culture conditions are: 35°C, 150r / min shaking culture for 36 hours;
[0056] 2) Frozen crushing treatment
[0057] Freeze the washed fish scales at -7...
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