Method for preparing oil in auxiliary cold pressing manner by solid-state fermentation of oil crops by two steps via aspergillus oryzae

Abstract

The present invention is a process for extracting oil from soybeans and other oil crops by solid-state fermentation and cold pressing using two-step method of Aspergillus oryzae. After the moisture content is reached, the logarithmic phase seed liquid or spore suspension of the bacteria is inserted into the solid substrate, and the oil is dissociated in two stages: the first stage of solid-state fermentation for enzyme production and enzyme activity determination, and the second stage of solid-state enzymatic release of oil Ingredients, the fermented material is vacuum-dried, and put into a cold press to extract oil. With the help of the growth of Aspergillus oryzae mycelium, the present invention allows the enzyme system of the bacteria to infiltrate and act on the tissue cells of oil crops such as soybeans, destroying its cellulose skeleton, disintegrating the lipoprotein complex completely, breaking down the tissue structure of oil crops, and making oil fully release, which greatly improves the oil yield of cold pressing of oil crops. The method has the characteristics of convenient operation, high oil yield of raw materials, no organic solvent residue, safe oil quality and the like.

Description

technical field [0001] The invention relates to a method for extracting vegetable oil, in particular to a method for extracting oil from soybeans and other oil crops by cold pressing after solid-state fermentation and enzymolysis by microorganism Aspergillus oryzae. Background technique [0002] The current conventional industrial oil production methods continue to use the traditional hot pressing process and organic solvent extraction. Although these two oil extraction processes have high oil extraction efficiency, they all have their own disadvantages. Steaming and frying are used in hot pressing, which leads to the maximum denaturation of high-quality protein, wastes a lot of protein resources, and indirectly increases the production cost. Although the oil extraction rate of organic solvent extraction is high, the chemical solvent used for oil extraction will A small amount remains in the refined oil and cake, and long-term consumption may cause certain harm to human heal...

AI Technical Summary

Problems solved by technology

At present, there are relatively few studies on the oil extraction technology combined with solid-state fermentation and cold pressing, especially the research on the influence of different strains on this process needs to be further explored and improved. Finding the appropriate strain is the key to affect the oil extraction effect and the quality of by-products

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