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Extracting process of pulullan polysaccharide

A technology of pullulan polysaccharide and extraction process, which is applied in the field of industrialized production of pullulan polysaccharide, can solve problems such as high cost, flocculant, organic solvent residue, and immature industrial extraction process route, and achieve good quality and safe production process Reliable, effective in reducing production costs

Active Publication Date: 2013-06-26
天津慧智百川生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our country started to carry out relevant research in the early 1980s, and now some papers and related patents have been published, and a few manufacturers have carried out industrial production, but the overall is still in the research stage, and the industrial extraction process route is not mature enough
The bottleneck of extraction technology mainly lies in the use of flocculants (such as polyaluminum chloride, polyferric chloride, etc.) and the addition of organic solvents (such as ethanol, methanol, isopropanol, etc.) It may cause the residue of flocculant and organic solvent, and there are potential safety hazards

Method used

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  • Extracting process of pulullan polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Removal of bacteria: make the viscosity 900mm 2 / s fermentation broth is diluted with deionized water twice, every 100mL of diluted fermentation broth is added with 0.7g of 100-mesh diatomaceous earth, pre-coated with 300-mesh diatomite with a 500-mesh filter cloth, and filtered with a plate-and-frame filter. To 0.22Mpa, remove the bacteria.

[0038] (2) Removal of protein: adjust the pH value of the fermented broth after removing the bacteria to 6.7 with a NaOH solution with a concentration of 5mol / L, keep it at 80°C for 20min, cool to room temperature, add 0.5g of 60 per 100mL of fermented broth Mesh powder activated carbon, carry out static adsorption for 1h, then use 700 mesh filter cloth to pre-coat 300 mesh diatomite, filter with plate and frame filter, the operating pressure is 0.20Mpa, remove the added activated carbon or the mixture of diatomite and activated carbon and Denatured protein flocs.

[0039] (3) One-time ultrafiltration desalination and concen...

Embodiment 2

[0046] (1) Removal of bacteria: make the viscosity 900mm 2 / s fermented liquid is diluted with deionized water twice, every 100mL of diluted fermented liquid is added with 100 mesh diatomite 1g, 600 mesh filter cloth is used to pre-coat 300 mesh diatomite, and a plate and frame filter is used for filtration. The operating pressure is 0.22Mpa, remove bacteria.

[0047] (2) Removal of protein: adjust the pH value of the fermented broth after removing the bacteria to 6.7 with a NaOH solution with a concentration of 5mol / L, keep it at 130°C for 20min, cool to room temperature, add 0.5g of 60 per 100mL of fermented broth Mesh powder activated carbon, carry out static adsorption for 1h, then use 700 mesh filter cloth to pre-coat 300 mesh diatomite, filter with plate and frame filter, the operating pressure is 0.20Mpa, remove the added activated carbon or the mixture of diatomite and activated carbon and Denatured protein flocs.

[0048] (3) One-time ultrafiltration desalination an...

Embodiment 3

[0055] (1) Removal of bacteria: make the viscosity 300mm 2 / s fermentation broth was diluted 0.5 times with deionized water, 1g of 100 mesh diatomite was added to every 100mL diluted fermentation broth, 600 mesh filter cloth was used to pre-coat 300 mesh diatomite, and a plate and frame filter was used for filtration. The operating pressure was 0.24Mpa, remove bacteria.

[0056] (2) Removal of protein: adjust the pH value of the fermented broth after removing the bacteria to 6.7 with a NaOH solution with a concentration of 5mol / L, keep it at 130°C for 20min, cool to room temperature, add 0.5g of 250 per 100mL of fermented broth Mesh powdered activated carbon and 0.5g of 100 mesh diatomite, conduct static adsorption for 1h, then use 700 mesh filter cloth to pre-coat 300 mesh diatomite, filter with plate and frame filter, operating pressure is 0.20Mpa, remove the added silicon A mixture of algal earth and activated carbon and denatured protein flocs.

[0057] (3) One-time ultr...

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Abstract

The invention discloses an extracting process of pulullan polysaccharide and belongs to the technical field of biology. The extracting process mainly comprises the following steps of: (1), filtering by a plate frame to remove bacteria; (2), performing thermal treatment to remove protein; (3), carrying out primary ultra-filtration; (4), removing ion by use of ion exchange resin; (5), discoloring active carbon; (6), carrying out secondary ultra-filtration; and (7), drying, crushing and packaging. According to the extracting process of the pulullan polysaccharide disclosed by the invention, the bacteria in fermentation liquor are removed by adopting a plate frame filter, so that not only can solvent residue caused by adding an organic flocculating agent and inorganic flocculating agent be effectively avoided, but also the bacteria and diatomite can be recycled to obtain more benefits; meanwhile, potential unsafe factors caused by volatility of solvents (ethanol, methyl alcohol, isopropanol and the like) in the conventional process can be avoided by adopting a microwave drying process or a vacuum drying process; moreover, much steam consumed by recycling and distilling an organic solvent can be saved; the production cost is saved; the product yield can be up to 90%-96%, the purity is as high as 90%-98%, the quality is good, and the production process is safe, continuous and automatic.

Description

technical field [0001] The invention relates to the industrial production technology of pullulan polysaccharide, which belongs to the field of biotechnology, in particular to an extraction process of pullulan polysaccharide. Background technique [0002] Pullulan is an extracellular water-soluble macromolecular neutral polysaccharide produced by Aureobasidium pullulans through sugar metabolism during the culture process. Its chemical structure is polymaltotriose linked by α-1,6-glycosidic bonds, that is, glucose is combined into maltotriose by α-1,4-glycosidic bonds, and the two ends are linked by α-1,6-glycosidic bonds Combined with another maltotriose, so repeatedly linked high molecular polysaccharide. Due to the different strains used, fermentation pH value, culture process conditions and substrates, the molecular weight of pullulan has a wide distribution range, which can be 2.0×10 5 -1.0×10 7 between daltons. Pullulan polysaccharide has excellent viscoelasticity, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 乔长晟高璇璇姜少丽白云宗
Owner 天津慧智百川生物工程有限公司
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