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Method for simultaneously determining five organic acid components content in Ixeris sonchifolia Hance injection by HPLC-DAD method

A technology of Kudiezi and injection, which is applied in the field of component analysis of traditional Chinese medicine, can solve the problems of poor specificity of ultraviolet method and high requirements for instruments, and achieve the effect of good stability, good repeatability and accurate results

Active Publication Date: 2013-06-19
TONGHUA HUAXIA PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The specificity of the ultraviolet method is poor, and the potentiometric titration method has higher requirements on the instrument

Method used

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  • Method for simultaneously determining five organic acid components content in Ixeris sonchifolia Hance injection by HPLC-DAD method
  • Method for simultaneously determining five organic acid components content in Ixeris sonchifolia Hance injection by HPLC-DAD method
  • Method for simultaneously determining five organic acid components content in Ixeris sonchifolia Hance injection by HPLC-DAD method

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Experimental program
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Embodiment

[0032] Method for simultaneous determination of five organic acids in Kudiezi injection by HPLC-DAD:

[0033] 1 Instruments and reagents

[0034] 1.1 Instrument

[0035]Waters-e2695-HPLC system (including quaternary gradient pump, online vacuum degasser, autosampler, column thermostat, 2998 photodiode array detector PDA, Empower 2 chromatography workstation); Sartorius BT125D analytical balance (Sartorius Sri Lanka Scientific Instrument (Beijing) Co., Ltd.); KH 2200B Ultrasonic Cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.); BT125D Analytical Balance (Germany Sartorius Company)

[0036] 1.2 Reagents

[0037] Monocaffeoyl tartaric acid reference substance (Shanghai Yixing Technology Co., Ltd., batch number: 111101); caffeic acid reference substance (Shanghai Yixing Technology Co., Ltd., batch number: 10112201); chlorogenic acid reference substance (Shanghai Yixing Technology Co., Ltd., batch number : 11042802); ferulic acid reference substance (National Institute...

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Abstract

The invention relates to a method for simultaneously determining five organic acid components content in a Ixeris sonchifolia Hance injection by HPLC-DAD method, which comprises the following steps: 1)preparing a mixed reference substance solution; 2)preparing a tested object solution; 3) using a HPLC-DAD method, wherein the chromatogram condition is characterized in that a chromatographic column is Diamonsil c18 (2), the column temperature is 40 DEG C, the detection wavelength is 325nm, the flow velocity is 1mL.min<-1>, the sample size is 10mumL; the mobile phase is acetonitrile and 0.1% of formic acid water, the gradient elution condition is characterized in that 9% of acetonitrile linearity is increased to 11% for 0-15 minutes, and is increased to 18% for 15-16 minutes, is increased to 20% for 16-30 minutes, and then increased to 49% for 30-40 minutes. The analysis method of five organic acid components content in Ixeris sonchifolia Hance injection has the advantages of rapid determination, good repeatability, accurate result and good stability, and can be used for controlling the quality of the Ixeris sonchifolia Hance injection.

Description

[0001] technical field [0002] The invention relates to a method for simultaneously determining the contents of five organic acid components in Kudiezi injection by HPLC-DAD, belonging to the field of component analysis of traditional Chinese medicines. Background technique [0003] In the prior art, Kudiezi has another name called Gypsophila gypsophila, and it is mainly distributed in Northeast my country and Inner Mongolia. Grass, cold in nature, bitter and pungent in taste, has the effects of clearing heat and detoxifying, draining pus and relieving pain [1] . Kudiezi (Diemailing) Injection is an intravenous injection made from Kudiezi extracted and refined, which has the functions of promoting blood circulation, removing blood stasis and improving microcirculation [2] . The injection has been on the market for many years, and its curative effect is definite. Experimental studies have shown that the chemical composition of Kudiezi injection is relatively complex, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 刘睿孙璐赵雯李遇伯张艳军
Owner TONGHUA HUAXIA PHARMA
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