Method for extracting saikosaponin from bupleurum chinense by using two-level foam separation process
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A foam separation method and foam separation technology, which is applied in the field of extraction and separation of total saponins of Bupleurum, can solve the problems of resin regeneration, large consumption of organic reagents, poor separation effect, etc.
Inactive Publication Date: 2013-06-12
XIAMEN UNIV
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[0006] The purpose of the present invention is to provide a two-stage foam separation method for extracting chai glucoside for the problems of large consumption of organic reagents, serious environm...
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Embodiment 1
[0028] The first step, preparation and detection of saikosaponin crude extract
[0029] Dry and pulverize 100g Bupleurum decoction pieces, pass through a 40-mesh sieve, cold soak and extract with ethanol with a pH of 9 and a concentration of 60% for 12 hours, and the ratio of solid to liquid is 1:7; filter, and extract twice by cold soak according to this process, and combine About 1.4L of the extract was stored in a refrigerator at 4°C; the concentration of the saikosaponin crude extract was 0.08-0.32 mg / ml, and the determination method was p-dimethylaminobenzaldehyde-hydrochloric acid colorimetric method.
[0030] The second step, Bupleurum crude extract pretreatment
[0031] The ethanol in the stock solution was recovered under reduced pressure to a volume of 200ml, and diluted to 2L with water to obtain a crude saikosaponin extract. The measured total saponin concentration of Bupleurum is 0.15mg / ml, the pH value is adjusted to 10, and the obtained saponin aqueous solution...
Embodiment 2
[0039] The first step, the preparation and detection of saikosaponin crude extract;
[0040] Dry and crush 70g Bupleurum decoction pieces, pass through a 40-mesh sieve, and extract with deionized water adjusted to pH 9 with sodium hydroxide for 12 hours by cold immersion method, the ratio of solid to liquid is 1:5; filter, and extract by cold immersion according to this process 2 times, the combined extracts were stored in a refrigerator at 4°C.
[0041]The second step, Bupleurum crude extract pretreatment
[0042] Take out the stock solution and add water to quantify it to 1L to obtain the saikosaponin crude extract. The measured total saikosaponin concentration is 0.18 mg / ml, adjust the pH value to 9, and use the obtained saponin aqueous solution as the initial feed liquid to enter the secondary foam The first stage foam separation tower in the separation process.
[0043] The third step, the first stage foam separation process
[0044] The temperature of the first-stage ...
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Abstract
The invention discloses a method for extracting saikosaponin from bupleurum chinense by using a two-level foam separation process, which relates to an extraction and separation method of the saikosaponin. The method comprises the following steps: drying and crushing the bupleurum chinense decoction pieces; extracting by using a solvent cold-soaking method; filtering to obtain the coarse extract liquid of the saikoside; recycling the solvent from the coarse extract liquid; separating the obtained saponin aqueous liquid serving as an initial liquid into a first-level foam separation tower; crushing foams of a foam layer, and stopping charge of gas when the foam cannot flow out of the top of the foam separation tower, wherein the foam which is crushed and flows out of the top of the foam separation tower is adopted as a first-level de-foaming liquid; separating the residual liquid in the tower which is adopted as the feeding liquid of the second-level foam separation in a second-level foam separation tower, wherein the foam flowing out of the top of the tower after being crushed is adopted as a second-level de-foaming liquid; stopping charge of gas when the foam cannot flow out of the top of the foam separation tower; mixing the second-level de-foaming liquid with the coarse extract liquid of saikoside, and then adding the mixture into the feeding liquid of the first-level foam separation tower; evaporating the de-foaming liquid in vacuum to obtain the concentrated liquid; and adjusting pH value to be subalkaline, freezing and drying to obtain the product.
Description
technical field [0001] The invention relates to a method for extracting and separating total saponins of Bupleuri, in particular to a method for extracting total saponins in Bupleuri by using a two-stage foam separation method. Background technique [0002] Bupleurum bupleuri is a perennial herb of Umbelliferae, which is widely used in Chinese patent medicine preparations because of its physiological activities such as anti-inflammatory, liver protection, lowering cholesterol and blood lipids. Modern studies have found that Bupleurum extract has anti-oxidation and mucopolysaccharide inhibition effects, and can also inhibit the formation of melanin and remove dark spots, so it can be used as an important component of anti-aging cosmetics and skin-brightening cosmetics. The main active ingredients of Bupleuri are volatile oil and saponin, as well as trace amounts of flavonoids, and saikosaponin is one of the important active ingredients. How to extract and separate its active ...
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