Preparation method of esterified red yeast fermented glutinous rice wine
A technology for esterifying monascus mash and Monascus, which is applied in the field of preparing mash by using esterified red yeast rice to achieve the effect of mellow taste
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Embodiment 1
[0034] A preparation method of esterified red yeast rice, comprising the following steps:
[0035] Strain mutagenesis:
[0036] The starting strains are: Monascus sooty strain, Monascus purple strain, Monascus orange strain; using the combination of cell fusion method and ultraviolet irradiation, the three starting strains were cultivated until the bacterial age was 35 hours, and the cytoplasmic decomposition was carried out. For: 28°C, time 2.8 hours, massolysis enzyme system including 2% lysozyme, 0.6% helicase and 0.6% cellulase, the massolysis of Monascus protoplasts at a distance of 25 cm under a 15w ultraviolet lamp, irradiated for 100 seconds , so that the fatality rate reaches about 91%;
[0037] Separation:
[0038] Separate the strains after mutagenesis on a plate, the plate medium is as follows, peptone 0.9%, polyvinyl alcohol olive oil emulsion 2.5%, sodium chloride 0.6%, agar 3%, after 72 hours of cultivation at 30°C, select the colony transparent circle Larger...
Embodiment 2
[0050] A preparation method of esterified red yeast rice, comprising the following steps:
[0051] Strain mutagenesis:
[0052] The starting strains are: Monascus sooty strain, Monascus purple strain, Monascus orange strain; using the combination of cell fusion method and ultraviolet irradiation, the three starting strains were cultivated until the bacterial age was 30 hours, and the cytoplasmic decomposition was carried out. For: 25°C, time 2.5 hours, massolytic enzyme system including 0.1% lysozyme, 0.3-1% helicase and 0.3% cellulase, the massolyzed Monascus protoplasts were irradiated at a distance of 25 cm from a 15w ultraviolet lamp 80 seconds, the fatality rate reaches 91%;
[0053] Separation:
[0054] Separate the strains after mutagenesis on a plate, the plate medium is as follows, peptone 1.2%, polyvinyl alcohol olive oil emulsion 3%, sodium chloride 1%, agar 5%, after 680 hours of culture at 32°C, select the colony transparent circle Larger single colonies should...
Embodiment 3
[0065] A preparation method of esterified red yeast rice, comprising the following steps:
[0066] Strain mutagenesis:
[0067] The starting strains are: Monascus sooty strain, Monascus strain purple, and Monascus orange strain; by combining cell fusion method and ultraviolet irradiation, the three starting strains are cultivated until the bacterial age is 30-40 hours, and the cytoplasm is decomposed. The solution conditions are: 30°C, time 3 hours, the massolytic enzyme system includes 5% lysozyme, 1% helicase and 0.8% cellulase, and the massolyzed Monascus protoplasts are irradiated at a distance of 25 cm from a 15w ultraviolet lamp. 100 seconds, the fatality rate is about 91%;
[0068] Separation:
[0069] Separate the strains after mutagenesis on a plate, the plate medium is as follows, peptone 1.2%, polyvinyl alcohol olive oil emulsion 3%, sodium chloride 1%, agar 5%, after 80 hours of culture at 32°C, select the colony transparent circle Larger single colonies should ...
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