Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Shark glycoprotein, and preparation method and application thereof

A technology of glycoprotein and shark, which is applied in the field of shark glycoprotein and its preparation method and anti-tumor application, achieving the effect of broad market prospect, abundant raw materials, and simple preparation process

Active Publication Date: 2013-05-22
ZHEJIANG OCEAN UNIV
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish meat is mainly used as a food supplement, but its active ingredients, especially the preparation process of fish meat glycoprotein and its application as an anti-tumor substance have not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Shark glycoprotein, and preparation method and application thereof
  • Shark glycoprotein, and preparation method and application thereof
  • Shark glycoprotein, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0031] Instruments and equipment used in the present invention:

[0032] Agilent 1200 HPLC (Agilent, USA)

[0033] Milli-Q system (Millipore, USA)

[0034] DEAE-cellulose-52 and Sephadex G-100 column chromatography equipment (Sweden Pharmacia company);

[0035] FDU-1200 Small Freeze Dryer (Japan EYELA Company)

[0036] DS-21 high-speed tissue grinder (Shanghai Specimen Model Factory);

[0037] UV-22000 ultraviolet spectrophotometer (Unico Instrument (Shanghai) Co., Ltd.);

[0038] DYY-22C electrophoresis instrument (Beijing Liuyi Instrument Factory);

[0039] Hei-VAP rotary evaporator (German Heidolph company);

[0040] BSZ-100 automatic part collector (Shanghai Qite Analytical Instrument Co., Ltd.)

[0041] LXB centrifuge (Shanghai Medical Analytical Instrument Factory);

[0042] Reagents and raw materials used in the present inventio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a shark glycoprotein, and a preparation method and a use thereof. The preparation method of the shark glycoprotein comprises the following steps: carrying out tissue triturating of shark meat, degreasing, carrying out enzymatic hydrolysis, purifying by allowing the obtained material to undergo DEAE-cellulose-52 column chromatography, Sephadex G-100 column chromatography and reversed-phase high-performance liquid chromatography, condensing, and lyophilizing to obtain the shark glycoprotein. The shark glycoprotein has a molecular weight of about 26.4kDa, a sugar content of 33.65% and a protein content of 66.35%, and contains an O-glycopeptide bond and an N-glycopeptide bond, the monosaccharide of the shark glycoprotein is composed of L-fucose, L-arabinose, L-galactose, D-glucose and D-mannose, and the L-fucose / L-arabinose / L-galactose / D-glucose / D-mannose ratio of 1.00:1.53:7.27:9.07:2.09; and the protein of the shark glycoprotein is composed of seventeen amino acids. The shark glycoprotein can be used as a neovascular inhibitor clinically, can be used for treating diseases comprising tumors and the like, and has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceutical engineering, and in particular relates to a shark glycoprotein, its preparation method and its application in antitumor. Background technique [0002] Glycoprotein is a kind of binding protein formed by linking carbohydrates (mostly oligosaccharides) and proteins or polypeptides with covalent bonds (O-glycosidic bonds or N-glycosidic bonds), and widely exists in animals, In plants and microorganisms, it is a very important class of functional macromolecules in organisms. Since the 1960s, after extensive and in-depth research, researchers in the fields of biochemistry, chemistry, and pharmacy have found that glycoproteins have significant medicinal and health value, and have anti-tumor, immune-enhancing, blood-lipid-lowering, and anti-inflammatory properties. Various functions such as diabetes, anti-oxidation and anti-aging. At present, most of the pharmaceutical protein preparations th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12P21/06C07K1/20C07K1/18C07K1/16A61K38/17A61P35/00A61P35/02
Inventor 王斌李忠瑞罗红宇邓尚贵马佳卉栗丽
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com