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Iridoids, and preparation method and application thereof

A technology for iridoids and compounds, applied in the field of iridoids and their preparation, to achieve the effect of strong anti-inflammatory activity

Active Publication Date: 2013-05-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Literature survey shows that the research on the components of gentian is mainly focused on gentian (G. scabra Bge.), and there are few reports on the chemical components and anti-inflammatory activity of gentian (G. triflora Pall.)

Method used

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  • Iridoids, and preparation method and application thereof
  • Iridoids, and preparation method and application thereof
  • Iridoids, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Extraction and separation of compound I and compound II

[0044] (1) Extraction and separation

[0045] See the extraction process figure 2 ,Specific steps are as follows:

[0046] 1) Take 6.5kg of dried Gentiana rhizome powder, add 6 times the volume of 80% ethanol solution, soak for 48h, perform percolation, and get the initial liquid;

[0047] 2) The filter residue was continuously percolated and extracted with 80% ethanol solution for 35 hours, and the flow rate was 17~34mL / min to obtain the continuous liquid;

[0048] 3) Combine the initial liquid and the continued liquid, and concentrate to a concentrated extract under reduced pressure at 40°C;

[0049] 4) The concentrated extract is diluted with 1.5 times the amount of water to obtain the extract. According to the extract: ethyl acetate about 1:1 (V / V) extraction six times, the ethyl acetate phase after extraction is decompressed at 38°C Concentrate to dryness to obtain 336g of extract;

[0050] 5) The obtained e...

Embodiment 2

[0070] Example 2 Anti-inflammatory activity of compound I and compound II

[0071] (1) Medicines, reagents and instruments

[0072] 1) The compound isolated above: (1S, 5R, 9R)-desglucosyl trilobatin, (1S, 5R, 9R)-gentioside;

[0073] 2) Mouse macrophages RAW264.7;

[0074] 3) Lipopolysaccharide (LPS), DMEM high glucose medium, DMEM phenol red-free medium, inactivated fetal bovine serum, double antibodies, DMSO, using Biyuntian Institute of Biotechnology Nitric Oxide Detection Kit (No. S0021);

[0075] 4) CO 2 Incubator, inverted microscope, clean bench, plate shaker, Bio-Tek microplate reader (ELX800).

[0076] (2) Experimental method

[0077] 1) Stimulate mouse macrophages RAW264.7 with 200ng / mL lipopolysaccharide to construct an inflammatory cell model;

[0078] 2) Inoculate the cells in a 96-well plate with a seeding density of 2×10 4 / Hole, after inoculation is completed in CO 2 Cultivate for 24h in an incubator;

[0079] 3) Replace the culture medium with DMEM phenol red-free medium c...

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PUM

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Abstract

The invention discloses iridoids, and a preparation method and application thereof. The structural formula of the iridoids is disclosed as Figure 1. The preparation method comprises the following steps: leaching Gentiana triflora Pall. in an organic solvent to obtain a leaching solution; extracting the leaching solution, and concentrating the extracting solution to obtain an extract; and separating and purifying the iridoids from the extract. The iridoids are applicable to preparing anti-inflammatory agents. Compared with the prior art, the iridoids disclosed by the invention are new compounds having strong anti-inflammatory activity, and can be used for preparing anti-inflammatory agents.

Description

Technical field [0001] The invention belongs to the field of phytochemistry, and particularly relates to an iridoid compound and a preparation method and application thereof. Background technique [0002] There are about 400 species of Gentian in the world, which are widely distributed in many countries in the northern hemisphere. They are distributed in mainland China, Russia, North Korea, Japan, etc., and grow in areas between 400 and 1700 meters above sea level. There are more than 240 species of gentian plants in China, which are mostly produced in the high mountains of southwest China. Together with rhododendrons and primulas, they are known as the world's three largest alpine flowers. [0003] According to the 2010 edition of the Pharmacopoeia, gentian belongs to the genus Gentianaceae: Gentiana scabra Bunge, G. triflora Pall., G. manshurica Kitag. Dry roots and rhizomes of G. rigescens Franch. "Compendium of Materia Medica" records: "Bitterness in nature and taste, ceres, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08A61P29/00
Inventor 程翼宇王书芳王毅
Owner ZHEJIANG UNIV
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