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Anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof

A denitrifying phosphorus-accumulating bacteria, nitrogen and phosphorus removal technology, applied in the cultivation of anaerobic denitrifying phosphorus-accumulating bacteria and its application field, can solve the problems of no identification method, no convenient and quick cultivation and domestication method, etc., to achieve complete denitrification Air, high phosphorus removal activity, no air pollution effect

Active Publication Date: 2013-05-15
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on this type of bacteria and their characteristics is still in its infancy, there is no systematic identification method, and there is no convenient and quick way of cultivating and domesticating

Method used

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  • Anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof
  • Anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof
  • Anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Isolation and identification of bacterial strains of the present invention:

[0036] (1) Medium:

[0037] A. Strain isolation, purification, preservation medium ( / L):

[0038]CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; Agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0~7.2.

[0039] B. Strain screening, denitrifying medium (DM: Denitrifying Medium) ( / L):

[0040] CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0~7.2.

[0041] C. LB medium ( / L):

[0042] Peptone, 10g; yeast extract, 5g; sodium chloride, 10g; pH is controlled at 7.5.

[0043] (2) Isolation and purification of Paenibacillus brilliantis CL-5 strain:

[0044] Separation and purification were carried out by dilution coating method and plate streaking method. First, use a sterile pipette to take 10 mL of the sludge mixture solution at the end of SBR hypox...

Embodiment 2

[0063] Embodiment 2 The cultivation of bacterial strain of the present invention

[0064] (1) Medium used

[0065] A. Strain preservation medium ( / L): peptone, 5g; yeast extract, 3g; glucose, 1g; NaCl, 6g; agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0~7.2.

[0066] B. Strain denitrification medium (DM: Denitrifying Medium) ( / L): CH 3 COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0~7.2.

[0067] The above medium was sterilized at 121°C for 20 minutes before use.

[0068] (2) Culture conditions

[0069] Use an inoculation loop to scrape a ring of Paenibacillus splendor CL-5 preserved on the slant of the preservation medium, inoculate it into a 250mL Erlenmeyer flask containing 100mL of sterilized LB medium, and culture it with constant temperature and shaking at 30°C After 12 hours, the seed liquid can be obtained. During the experiment, the seed liquid was inocula...

Embodiment 3

[0070] Example 3 The optimal denitrification conditions of the bacterial strain of the present invention

[0071] Put 100mL of sterilized denitrification medium into a 250mL Erlenmeyer flask, insert the seed bacteria solution according to the inoculation amount of 10% (v / v), and let it stand for cultivation. Its optimal denitrification temperature is between 25-35°C, and the optimum temperature is 30°C. The pH of the strain is between 7 and 8, and it has high-efficiency denitrification activity. The growth cycle of the bacteria is long, entering the logarithmic growth phase after 48h, entering the stable phase after 96h, basically in the stable phase from 96 to 144h, and entering the decline phase after 144h. The nitrogen and phosphorus removal rates of the strain were 57.5% and 50.2% in the first 24 hours, and the nitrogen and phosphorus removal rates in the whole growth cycle were 72.3% and 64.1%, respectively.

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Abstract

The invention relates to an anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof. The strain is named as Paenibacillus lautus CL-5, belongs to bright bacillus CL-5 and has the collection number of CCTCC NO: M2012227. The strain is separated and screened from sludge of a double sludge sequencing batch type reactor (DSSBR) when anoxia is ended. The strain is long in growth period and enters the logarithmic phase within 48 hours, the nitrogen and phosphorus removal rate of the strain is respectively 57.5 percent and 50.2 percent in the previous 24 hours, and the nitrogen and phosphorus removal rate in the whole growth period is respectively 72.3 percent and 64.1 percent. The final denitrification product is identified into nitrogen, so that the strain has complete denitrification capacity, and the nitrate nitrogen, nitrite nitrogen and other inorganic nitrogen in the water body can be directly reduced into harmless nitrogen to be discharged out of the water body. The performance detection of the strain proves that the process for treating the nitrogen-containing wastewater by employing the strain is simple, the treatment effect is high-efficiency and stable, NO, N2O and other greenhouse gas are not generated, and air pollution is avoided.

Description

technical field [0001] The invention relates to the cultivation and application of an anaerobic denitrifying phosphorus-accumulating bacterium capable of denitrification and phosphorus removal, and belongs to the field of environmental microorganisms. Background technique [0002] With the rapid development of my country's economy, water pollution has become a major concern of all sectors of society. At present, total nitrogen and total phosphorus are the most harmful to water bodies. A large amount of nitrogen and phosphorus entering the water will cause a sharp deterioration of the water environment. The main harm lies in the eutrophication of the water body. increased to 55%, while the proportion of oligotrophic water bodies decreased from 3.2% to 0.53%. The eutrophication of the water body promotes the massive reproduction of algae. After the excessive reproduction, a large number of algae decay and die, which provides a rich substrate for the growth of heterotrophic mi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/28C02F3/34C12R1/01
Inventor 裴海燕李政胡文容邵媛媛孟盼盼
Owner SHANDONG UNIV
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