Method for producing single-cell protein feed by utilizing bean product waste water
A single-cell protein, soybean product wastewater technology, applied in animal feed, animal feed, application, etc., can solve the restriction of popularization and application, waste of water-soluble protein, oil, total sugar, water-soluble vitamins, and reduce the COD quality of soybean product wastewater Concentration and other issues to achieve the effect of reducing environmental pollution
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Embodiment 1
[0025] The first step, production and seed production Transfer the Geotrichum candidum, Candida utilis, and photosynthetic bacteria that have been screened by the bacteria production test into eggplant bottles equipped with PDA agar medium, wort medium, and photosynthetic bacteria medium. , incubate at 30-35°C for 45-50 hours, take it out when the surface of the eggplant bottle is covered with a lawn, and turn white with yellow, and then store in a refrigerator at 2-6°C;
[0026] The second step, liquid high-speed culture of bacteria
[0027] (1) Weigh the bacteria cultured in step 1 according to the following weight percentages: 35% of Candida utilis, 25% of photosynthetic bacteria, and 40% of Geotrichum candidum;
[0028](2) Compound culture of strains: Put the strains weighed above into the medium with the following proportions at a ratio of 1:6: 15% molasses, 3% peptone, 4% corn flour, 0.5% sodium chloride, yeast Paste 0.5%, Magnesium Phosphate 0.05%, Potassium Dihydrogen...
Embodiment 2
[0035] The first step, production and seed production are the same as in Example 1
[0036] The second step, liquid high-speed culture of bacteria
[0037] (1) Weigh the bacteria cultured in step 1 according to the following weight percentages: 34% of Candida utilis, 22% of photosynthetic bacteria, and 34% of Geotrichum candidum;
[0038] (2) Compound culture of strains: put the strains weighed above into the medium with the following ratio at a ratio of 1:10: 15% molasses, 3% peptone, 4% corn flour, 0.5% sodium chloride, yeast Paste 0.5%, Magnesium Phosphate 0.05%, Potassium Dihydrogen Sulfate 0.05%, Sterile Water 77.4%. Put it into a fermentation tank for cultivation, the stirring speed is 200r / min-240r / min, the cultivation temperature is 28°C, the ventilation rate is 1:0.6, the fermentation time is 18-20 hours, and the fermentation ends when the pH value reaches 4.5-6;
[0039] The third step is soybean product wastewater deep liquid culture
[0040] Culture medium prepa...
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