Novel anti-tumor nano-drug carrier and preparation method and application thereof
A nano-drug carrier, anti-tumor technology, applied in the direction of anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problem that the toxic and side effects of anti-cancer drugs cannot be effectively reduced, the circulation time is not long enough, and the delivery carrier system is few. and other problems to achieve the effect of avoiding rapid phagocytosis, strong three-dimensional protection, and high flexibility
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Embodiment 1
[0058] (1) Hydrophobic modification of chitosan:
[0059] Weigh 1.0g chitosan in a reaction bottle, add 100ml of 1% (v / v) acetic acid aqueous solution to dissolve it, and obtain chitosan acetic acid solution; mix 0.3180g DA (chitosan (CS) and deoxycholic acid (DA) molar ratio is 1:40), 0.6211g EDC, 0.1243g NHS were added to another beaker, and 200ml methanol was added to activate for half an hour to obtain a mixed solution; then the mixed solution was added dropwise to the chitosan acetic acid solution, Magnetic stirring, react at 25°C for 48 hours, when the reaction is over, add 60ml of ammonia water to the reaction bottle, transfer the reactant to a centrifuge tube and centrifuge for 10 minutes (speed 5000r / min), discard the supernatant, wash the precipitate with 40ml of methanol 3 Then the precipitate was transferred to a dialysis bag (the molecular weight cut-off of the dialysis bag is 10000, the same below.) Dialyzed for 7 days, freeze-dried (freeze at -10°C overnight and...
Embodiment 2
[0073] (1) Hydrophobic modification of chitosan:
[0074] Weigh 1.0g chitosan in a reaction bottle, add 100ml of 1% (v / v) acetic acid aqueous solution to dissolve it, and obtain chitosan acetic acid solution; mix 1.2720g DA (chitosan (CS) and deoxycholic acid (DA) molar ratio is 1:162), 2.4844g EDC, 0.4971g NHS were added to another beaker, and 200ml methanol was added to activate for half an hour to obtain a mixed solution; then the mixed solution was added dropwise to the chitosan acetic acid solution, Magnetic stirring, react at 25°C for 60 hours, when the reaction is over, add 60ml of ammonia water to the reaction bottle, transfer the reactant to a centrifuge tube and centrifuge for 10 minutes (speed 5000r / min), discard the supernatant, wash the precipitate with 40ml of methanol 4 Then, the precipitate was transferred to a dialysis bag for dialysis for 7 days, and then freeze-dried (freeze at -10°C overnight and then freeze-dry in a freeze dryer for 24 hours) to obtain chi...
Embodiment 3
[0085] (1) Hydrophobic modification of chitosan:
[0086] Weigh 1.0g chitosan in a reaction bottle, add 100ml of 1% (v / v) acetic acid aqueous solution to dissolve it, and obtain chitosan acetic acid solution; mix 0.8480g DA (chitosan (CS) and deoxycholic acid (DA) molar ratio is 1:108), 1.6563g EDC, 0.3315g NHS were added to another beaker, and 200ml methanol was added to activate for half an hour to obtain a mixed solution; then the mixed solution was added dropwise to chitosan acetic acid solution, Magnetic stirring, react at 25°C for 72 hours, when the reaction is over, add 60ml of ammonia water to the reaction bottle, transfer the reactant to a centrifuge tube and centrifuge for 10 minutes (speed 5000r / min), discard the supernatant, wash the precipitate with 40ml of methanol 5 Then, the precipitate was transferred to a dialysis bag for dialysis for 7 days, and then freeze-dried (freeze at -10°C overnight and then freeze-dry in a freeze dryer for 24 hours) to obtain chitosa...
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