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Specific antibody against herbicide anilofos

A technology for sapron and herbicide, applied in the field of sap hapten, can solve the problems of high cost, complicated and complicated operation, slow analysis speed and the like, and achieve the effects of fewer reaction steps, simple synthesis method and easy acquisition.

Inactive Publication Date: 2014-07-02
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these traditional physical and chemical analysis methods are highly sensitive and accurate, they are difficult to meet the needs of actual analysis due to their complicated operation, high cost and slow analysis speed. Therefore, it is urgent to develop a simple, fast and sensitive method. analytical techniques

Method used

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  • Specific antibody against herbicide anilofos
  • Specific antibody against herbicide anilofos
  • Specific antibody against herbicide anilofos

Examples

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Embodiment 1

[0030] 1. Synthesis of N-chloroacetyl-N-isopropyl-4-chloroaniline (CCPA for short)

[0031] The present invention selects CCAP as the intermediate product of hapten synthesis, and its molecular structural formula is:

[0032]

[0033] The specific preparation method is: weigh 5.859g (0.035mol) of N-isopropyl p-chloroaniline, 28ml of anhydrous toluene, and 3.57g (0.035mol) of triethylamine in a round bottom flask, stir at room temperature, and use a constant 3.92 g (0.035 mol) of chloroacetyl chloride was added dropwise into the pressure drop funnel (control the rate of dropping to keep the temperature below 65° C.). After the dropwise addition, keep the temperature at 110°C and reflux for 5-6h. Cool, filter, wash the formed triethylamine hydrochloride with benzene, wash the filtrate with water until neutral, dry over anhydrous sodium sulfate, and filter to obtain a dark solution, which is identified by mass spectrometry. The product was purified through a silica gel colum...

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Abstract

The invention provides preparation of a semi-antigen and an artificial antigen and an antibody against herbicide anilofos, which relates to the semi-antigen, the artificial antigen and the antibody which have a basic structure of the herbicide anilofos. According to the invention, the antibody with good specificity to the herbicide anilofos is prepared by using an easy and convenient method. The antibody is prepared through the following steps: synthesizing the semi-antigen from N-chloracetyl-N-isopropyl-4-chloroaniline and mercaptoacetic acid; respectively connecting the semi-antigen with bovine serum albumin (BSA) and horseradish peroxidase (HRP) to synthesize the artificial antigen and an enzyme-labeled antigen; and subjecting the artificial antigen to animal immunization, blood drawing, separation of antiserum and purification so as to prepare the antibody. The antibody is stable and has good specificity and sensitivity; the synthetic method for the antibody is simple; and the antibody can be used for rapid immunodetection of residual herbicide anilofos in the environment, agricultural products and foodstuffs and has a good application prospect.

Description

technical field [0001] The present invention relates to selecting a compound with -COOH and possibly containing the original structure of saponin as the hapten of saponin, and making the hapten into an antigen to generate antibodies; and the hapten and antigen Synthesis and antibody production methods. The invention belongs to the field of biotechnology. Background technique [0002] Herbicide is a type of pesticide that has been gradually developed in the past 20 years. With the development of the chemical industry, the variety of herbicides has gradually increased. Dozens of herbicides have been developed and put into production in my country. Some herbicides have teratogenic effects, and the safety of herbicides has attracted people's attention. Attention should be paid to the environmental pollution and human and animal toxicity of herbicides. The use of herbicides can remain in grains, etc., and cause chronic harm to humans and animals through the food chain. In Jap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765C07K1/113C07K16/44
Inventor 王俊平王硕生威
Owner TIANJIN UNIV OF SCI & TECH
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