Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pre-treating extraction method for detecting melamine in soil and fertilizer

A technology for melamine and an extraction method is applied in the extraction field for pretreatment for determination of melamine in soil and fertilizers, and can solve the problems of less research on melamine extraction methods and poor extraction effect, etc.

Inactive Publication Date: 2013-04-17
上海出入境检验检疫局工业品与原材料检测技术中心 +1
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the detection method of melamine is mature, there are few studies on the extraction method of melamine, and the extraction effect of melamine in soil and fertilizer reported in some existing literature is not good, so the extraction method of melamine in soil and fertilizer is optimized , in order to truly measure the content of melamine in the above samples, which is of great significance to the quality safety and supervision of melamine in fertilizers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pre-treating extraction method for detecting melamine in soil and fertilizer
  • Pre-treating extraction method for detecting melamine in soil and fertilizer
  • Pre-treating extraction method for detecting melamine in soil and fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 The extraction effect of mechanical vibration is obviously better than that of ultrasonic vibration

[0016] 1) Ultrasonic extraction step: Weigh 1 g of four fertilizer samples (accurate to 0.1 mg) into a 50 mL centrifuge tube, add 25 mL of ammonia methanol solution, ultrasonicate for 30 min, centrifuge for 5 min, and pour the supernatant into another 50 mL centrifuge tube; Add 25mL ammonia water methanol solution again, sonicate for 30min, centrifuge for 5min, mix the two supernatants, take 10mL extract and blow dry with nitrogen, dilute to 5mL with diluent, pass through 0.45μm organic filter membrane, and detect by HPLC.

[0017] 2) Mechanical vibration extraction step: Weigh 1g of fertilizer sample into a 100mL Erlenmeyer flask, add 30mL ammonia water methanol solution, shake for 30min, centrifuge for 5min, pour the supernatant into a 50mL centrifuge tube; add 30mL ammonia water methanol solution again, shake 30min, centrifuge for 5min, mix the two supernat...

Embodiment 2

[0022] Example 2 The basic organic compound is obviously better than other water-soluble organic compounds as the extraction solution

[0023] In order to select a better extract, we took a certain amount of fertilizer samples and did the following experiments:

[0024] 1) Weigh 1g of chemical fertilizer sample, shake with 30mL 5 / 85 / 10 ammonia water / methanol / water, 30mL 50 / 50 methanol / water, 30mL water, and 30mL mobile phase (such as: 15% acetonitrile salt buffer) respectively, shake for 30min, Extract twice, take 10mL of nitrogen blowing, dilute to 5mL, pass through 0.45μm organic filter membrane;

[0025] 2) Weigh 1g of fertilizer sample, add 1mL 5ppm melamine standard solution, respectively use 30mL 5 / 85 / 10 ammonia water / methanol / water, 30mL 50 / 50 methanol / water, shake for 30min, extract twice, take 10mL nitrogen blowing, set Make up to 5mL, pass through 0.45μm organic filter membrane;

[0026] 3) Weigh 1g of fertilizer sample, add 1mL of 500ppm melamine standard solution...

Embodiment 3

[0040] Example 3 The effect of using a buffered organic solvent containing a small amount of salt as a diluent is obviously better than that of a single organic solvent.

[0041] In order to determine the composition of the diluent, we conducted the following experiments:

[0042] After the fertilizer samples were extracted according to the aforementioned optimal extraction method, 10 mL was taken, blown dry with nitrogen gas, and fixed to volume with 5 mL of 20% methanol aqueous solution and 5 mL of 15% acetonitrile buffer solution respectively. See the attachment for HPLC test results figure 1 .

[0043] Through the comparison of the melamine standard spectrum and the assignment of the peaks, we can clearly see that the detection sensitivity is greatly improved when using 15% acetonitrile buffer as the constant volume solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method which is scientific and reasonable, strong in operability, and wide in applicability, and according to the method, results are satisfying by means of addition standard recovery and standard control. The invention relates to a pre-treating extraction method for detecting melamine in soil and a fertilizer. According to the method, the best extraction condition for detecting the melamine in the soil and the fertilizer can be obtained by optimizing a plurality of parameters such as an extraction way, the composition of an extracting solution and the composition of a diluted solution, so that the content of the melamine in the sample is detected precisely, factually and reliably. The pre-treating extraction method can be applied to the detection of the melamine in the same product. The method is scientific and reasonable, strong in operability, and wide in applicability, and according to the method, the results are satisfying by means of the addition standard recovery and the standard control.

Description

1. Technical field [0001] The present invention relates to an extraction method used for the pretreatment of the determination of melamine in soil and fertilizers. The method has great innovations in the extraction method, selection of extraction solution and dilution solution, etc., and can truly and effectively determine the melamine in the above soil and fertilizer samples. The content of melamine, its extraction effect is very satisfactory. 2. Background technology [0002] Melamine, which is added to milk powder, pet food and animal feed, causing a series of human and animal deaths due to kidney failure, is an important nitrogen-heterocyclic organic chemical raw material, often used in the production of plastics, glue, textiles, etc. , leather, medicine and flame retardants are also used to make fertilizers in some Asian countries. At the same time, melamine is also a metabolite of a pesticide called cyromazine. Melamine is closely related to people's lives, and its bi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
Inventor 孙明星任盼盼王亭亭赵雨薇张继东屠虹高运川沈国清
Owner 上海出入境检验检疫局工业品与原材料检测技术中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com