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Method for initiating mammalian stem cells and application of chlorine dioxide in preparation of medicament for initiating mammalian stem cells

A technology of chlorine dioxide and mammals, applied in the field of regenerative medicine, can solve the problems of no obvious hair growth effect, no maximum ability of chlorine dioxide, no discovery of the mechanism of chlorine dioxide initiating stem cells, etc., and achieve small side effects and burden light effect

Active Publication Date: 2013-04-17
刘学武
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some patents related to the use of chlorine dioxide to treat some diseases (for example, CN102137651A, CN101641104A and CN1199633C), these patents still only use the strong oxidation ability of chlorine dioxide and the anti-infection ability to kill skin pathogenic microorganisms
Patent No. US5750108 mentions that chlorine dioxide can stimulate new hair follicle development, but due to the low concentration, the maximum ability of chlorine dioxide has not been brought into play, so there is no obvious hair growth effect; the patent publication No. CN102441006A provides a kind of The topical hair growth solution of chlorine dioxide can stimulate the growth of new hair in the hair loss area in a short period of time, and provides a curable case, but the patent still does not find the potential mechanism of action of chlorine dioxide to activate stem cells

Method used

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  • Method for initiating mammalian stem cells and application of chlorine dioxide in preparation of medicament for initiating mammalian stem cells
  • Method for initiating mammalian stem cells and application of chlorine dioxide in preparation of medicament for initiating mammalian stem cells
  • Method for initiating mammalian stem cells and application of chlorine dioxide in preparation of medicament for initiating mammalian stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0171] This example is used to illustrate that the stem cell activator containing chlorine dioxide is used as a medicine to treat androgen alopecia.

[0172] Drug preparation: Use deionized water to prepare a mixed solution of 7.47% sodium chlorite and 1.59% sodium chloride to prepare the first solution; use deionized water to prepare a 16.7% citric acid solution to prepare the first solution Two solutions.

[0173] Usage and dosage: Take out the same volume of solution from different solution containers, put them into the pre-prepared glass or plastic cups of appropriate size, wait for the solution to mix and stand still for 3 to 5 minutes, and then use 0.22μm double-layer filter membrane Filter, smear the hair loss area with a cotton swab dipped in liquid, wait for 1 to 2 hours, and wash the head with water once a day. Use according to the course of treatment, the first course of treatment is 15-25 days, the drug is stopped for 2 to 5 months, and then the second course of treatm...

Embodiment 2

[0187] In this example, a stem cell activator containing chlorine dioxide is used as a drug in patients suffering from a vascular disease-deep vein thrombosis of the lower limbs (lower limb DVT), to illustrate that the stem cell activator containing chlorine dioxide is used in peripheral blood CD34 positive cells, CD34 positive / CD31 positive cells and vascular endothelial cadherin positive cells (VE-Cadherin) positive cells in the priming effect, as well as the regenerative effect of stem cell promoters containing chlorine dioxide on blood vessels damaged by thrombus.

[0188] In addition, it is known that those cells that exist in peripheral blood and are considered to be CD 34-positive mononuclear cells are vascular EPC and mesenchymal stem cells (Zhao Y et al.: A human peripheral bloodmonocyte-derived subset acts as pluripotentstem cells. ProcNatlAcadSci USA.100:2426-31,2003). Therefore, it can be said that the CD34-positive mononuclear cells in the peripheral blood evaluated ...

Embodiment 3

[0246] In this example, a rat cerebral ischemia / reperfusion injury model was used to illustrate the effect of the chlorine dioxide-containing stem cell activator of the present invention on the activation of neural stem cells and the recovery of nerve function as a drug.

[0247] (1) Test object

[0248] Male rats aged 12 weeks and weighing 250-280 g were used for the test after acclimation for one week.

[0249] (2) Establish cerebral ischemia / reperfusion injury model

[0250] First, the middle cerebral artery occlusion model was established according to the method of Longa EZ et al. (Longa EZ et al.: Reversible middle cerebral artery occlustion without craniectomy in rats. Stroke 20:84-91, 1989). More specifically, 0.36 g / kg, 10% chloral hydrate was intraperitoneally administered to rats that had been fasted for 12 hours (but with free drinking water) to anesthetize the rats. Next, make a midline incision in the neck to expose the right common carotid artery. Then, the internal ca...

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Abstract

The invention provides a method for initiating proliferation, migration and differentiation of stem cells in the bodies of mammalians. The method comprises the steps of preparing a stem cell initiating agent containing chlorine dioxide and supplying the stem cell initiating agent to mammalian target tissues, wherein effective dose of chlorine dioxide is provided when the stem cell initiating agent is supplied to the mammalian target tissues. The invention also provides an application of the chlorine dioxide in preparation of a medicament for initiating proliferation, migration and differentiation of the stem cells. The method is wide in applicable range, and is suitable for initiating proliferation, migration and differentiation of the stem cells in bodies for treating diseases or promoting regeneration of injured organs or tissues by using the stem cell tissue regeneration capability and the immune regulating capability; moreover, proliferation, migration and differentiation of the stem cells can be quickly and properly initiated by adopting the method; and the method is low in side effect or does not have side effect, and can continually exert the effect of initiating proliferation, migration and differentiation of the stem cells.

Description

Technical field [0001] The invention belongs to the field of regenerative medicine, and specifically relates to a method for initiating the proliferation, migration and differentiation of stem cells in mammals, and the application of chlorine dioxide in the preparation of drugs for initiating the proliferation, migration and differentiation of stem cells. Background technique [0002] Human diseases can be roughly divided into organic diseases and functional diseases. In modern medicine, medical treatment is mainly for functional diseases, while surgical treatment and medical treatment can be used for organic diseases. However, most medical treatments are symptomatic treatment, and in many cases the disease cannot be cured. In addition, surgical treatment is more of an invasive treatment method, which is performed by removing all or part of the damaged organs, resulting in partial or complete loss of organ function. On the other hand, organ transplantation and artificial organs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/00A61P17/00A61P9/10A61P25/02A61P25/16A61P9/00A61P1/04A61P1/00A61P1/16A61P1/18A61P11/00A61P13/12A61P13/08A61P19/08A61P9/14A61P27/16A61P37/08A61P37/02A61P31/00
CPCA61K33/20A61P1/00A61P1/02A61P1/04A61P1/16A61P1/18A61P3/10A61P5/00A61P7/02A61P9/00A61P9/10A61P9/14A61P11/00A61P11/02A61P13/00A61P13/08A61P13/10A61P13/12A61P17/00A61P17/02A61P17/04A61P17/14A61P17/16A61P19/02A61P19/08A61P21/00A61P21/02A61P21/04A61P25/00A61P25/02A61P25/16A61P25/28A61P27/00A61P27/02A61P27/04A61P27/06A61P27/14A61P27/16A61P29/00A61P31/00A61P31/04A61P31/22A61P37/02A61P37/08A61P41/00A61P43/00
Inventor 刘学武
Owner 刘学武
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