Far infrared temperature jump microscope

Abstract

A far infrared temperature jump microscope comprises a laser light source portion, fluorescence optical filter components, a microscope imaging system, an objective optical system, and an objective table. The fluorescence optical filter components is arranged between the laser light source portion and the objective optical system. The fluorescence optical filter components comprise a transmitting optical filter, a dichroscope and an exciter filter. The objective optical system comprises a nosepiece and the objective fixedly arranged on the nosepiece. A far infrared laser system is arranged between the fluorescence optical filter components and the objective optical system, stimulates to produce far infrared laser and gathers the far infrared laser light on the test specimen. The far infrared temperature jump microscope is capable of conducting accurate heating of micro region of the biological sample specimen, and the heating efficiency is high.

Description

technical field [0001] The invention relates to a fluorescence microscope for observing or imaging biological cell tissues, in particular to a fluorescence microscope capable of heating cell tissues. Background technique [0002] Using heat to control exogenous gene expression and ion channel switching has a very wide range of applications in biomedicine. The heat shock protein promoter is a group of highly conserved polypeptides in structure. When the external temperature is high (greater than 35 degrees), the promoter is activated and regulates the expression of its downstream genes. This method is a very important experimental method in developmental biology. In the large family of ion channels, many temperature-sensitive ion channels have been discovered recently, but the research on their biological functions is still quite scarce. Controlling the switching of ion channels by means of local heating is the most effective and direct experimental method to study their fu...

AI Technical Summary

Problems solved by technology

[0004] (1) Unable to micro-area heating

Whether it is water bath or electric heating, it can only heat the biological specimen as a whole or a large area locally, and cannot precisely heat the micro-region (ten to tens of microns) or single cell (ten microns) of interest;

[0005] (2) Low heating efficiency and long heating time

Prolonged heating will cause greater damage to individual organisms and affect their biological activity;

[0006] (3) The temperature cannot be precisely controlled

Different tissues have different heat conductivities, and the heat conduction model is complex, so it is theoretically difficult to calculate the actual temperature gradient distribution in the heating area

At the same time, traditional methods cannot monitor the temperature in different areas in real time and adjust accordingly;

[0007] (4) Cannot be heated deeply

Due to the poor thermal conductivity of biological tissues, some internal tissues cannot be heated effectively

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