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Detection method of medicament for treating coronary disease and stenocardia

A detection method and technique for angina pectoris are applied in the detection of Gelanxinning soft capsules, soft capsules for angina pectoris, and in the field of treatment of coronary heart disease. The effect of improving quality and meeting medical needs

Inactive Publication Date: 2013-04-10
XIAN CHIHO PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing detection method of Gelanxinning Soft Capsules, the total flavonoids of Pueraria root and the total saponins of Gypenosides were identified by thin-layer chromatography (TLC), but the identification method of hawthorn extract by TLC was not established

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  • Detection method of medicament for treating coronary disease and stenocardia
  • Detection method of medicament for treating coronary disease and stenocardia
  • Detection method of medicament for treating coronary disease and stenocardia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Prescription and preparation method of Gelan Xinning soft capsule

[0042] 1. Prescription: 200g of total flavonoids of kudzu root, 60g of hawthorn extract, 20g of gypenosides, 254g of salad oil, 6g of beeswax, 20g of hydrogenated palm oil, 10g of soybean lecithin, 0.5g of methyl silicone oil, made into 1000 capsules.

[0043] 2. Preparation method: take salad oil, add beeswax and hydrogenated palm oil, heat to dissolve, let cool, add soybean lecithin, stir well, add kudzu root flavonoids, hawthorn extract, gypenosides, stir well, colloid mill to grind well , add methyl silicone oil, decompress and defoam, and press the medicine to make soft capsules.

Embodiment 2

[0044] Example 2 TLC Identification of Hawthorn Extract in Gelanxinning Soft Capsules

[0045]1. Test conditions: double-slot expansion cylinder (Shanghai Xinyi); silica gel G (for thin-layer chromatography, Qingdao Ocean Chemical Factory); sodium carboxymethyl cellulose (Sinopharm Chemical Reagent Co., Ltd.); self-made thin-layer plate, thickness 0.3mm. The rest of the reagents were analytically pure.

[0046] 2. Test procedure: Three different batches of Gelanxinning Soft Capsules (purchased from Xi'an Qianhe Pharmaceutical Co., Ltd., whose composition, proportion and preparation method are as shown in Example 1) were taken. Add 2 g of diatomaceous earth, grind finely, add absolute ethanol 50 ml, ultrasonically treat for 30 minutes, filter, the filtrate is concentrated to 5 ml, and the supernatant is taken as the test solution.

[0047] Take another ursolic acid reference substance, add absolute ethanol to make a solution containing 1mg per 1ml, as the reference substanc...

Embodiment 3

[0051] Example 3 TLC Identification of Hawthorn Extract in Gelanxinning Soft Capsules

[0052] 1. Test conditions: double-slot expansion cylinder (Shanghai Xinyi); silica gel G (for thin-layer chromatography, Qingdao Ocean Chemical Factory); sodium carboxymethyl cellulose (Sinopharm Chemical Reagent Co., Ltd.); self-made thin-layer plate, thickness 0.3mm. The rest of the reagents were analytically pure.

[0053] 2. Test procedure: Take 2g of the content of Gelanxinning soft capsule (purchased from Xi'an Qianhe Pharmaceutical Co., Ltd.), add appropriate amount of diatomaceous earth, grind it finely, add 50ml of absolute ethanol, ultrasonicate for 30 minutes, and filter , The filtrate was concentrated to 5ml, and the supernatant was taken as the test solution.

[0054] Take another ursolic acid reference substance, add absolute ethanol to make a solution containing 1mg per 1ml, as the reference substance solution.

[0055] According to the test of thin-layer chromatography ...

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Abstract

The invention provides a detection method of a medicament for treating coronary disease and stenocardia. The detection method comprises the step of identifying a hawthorn extract in the medicament by adopting a thin layer chromatography, wherein the conditions of the thin layer chromatography are that the mixed solution of a solution A, a solution B and a solution C which are in a volume ratio of (15-25):(2-6):(0.1-1.0), preferably 20: 4:0.5, is used as a developing agent, wherein the solution A is selected from benzene, methylbenzene, dichlorotoluene, chloroform and diethyl ether; the solution B is selected from ethyl acetate, ethyl formate, acetone, alcohol and methyl alcohol; and the solution C is selected from acetic acid, methanoic acid and glacial acetic acid. The detection method has the advantages of high sensitivity, good repeatability, no interference from negative control, and strong maneuverability in the whole testing process, and can be used as one index for controlling the inherent quality.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a method for detecting a drug for treating coronary heart disease and angina pectoris, in particular to a detection method for soft capsules for treating coronary heart disease and angina pectoris, such as the detection of Gelanxinning soft capsules method. Background technique [0002] Gelanxinning Soft Capsule is composed of total flavonoids of kudzu root, hawthorn extract, and total saponins of gypenosides, which have synergistic effects and are in line with the principles of traditional Chinese medicine prescriptions, and can achieve the goal of treating both symptoms and root causes. The preparation absorbs the essence of classic ancient prescriptions, has the effects of promoting blood circulation and removing blood stasis, dredging collaterals and relieving pain, and is used to treat coronary heart disease and angina pectoris caused by congestion and obstructi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCA61K36/734A61P9/10G01N30/90
Inventor 胡小虎吕慧锋赵宗平
Owner XIAN CHIHO PHARMA
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