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Staphylococcus cohnii selective medium and preparation method thereof

A staphylococcus, selective technology, applied in the field of medical testing, can solve the problems of inability to selectively cultivate Staphylococcus koridi and the inability to grow Staphylococcus koridi, and achieve the effect of reliable detection and high isolation rate

Inactive Publication Date: 2013-04-03
陈淑香
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this medium cannot selectively cultivate Staphylococcus koridi, the reason being that in the current selective medium, if other Staphylococci are mixed in the specimen, Staphylococcus aureus grows faster than Staphylococcus koridi, The medium is quickly covered and Staphylococcus coli cannot grow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; 0.5 g; Potassium dihydrogen phosphate 0.2 g; add distilled water to 1000ml. Preparation method: Dissolve malt extract powder; egg white liquid; sodium chloride; magnesium citrate; , sterilized twice at low temperature, 45 min each time, and packed after sterilization.

Embodiment 2

[0027] Example 2, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; 0.5 g; Potassium dihydrogen phosphate 0.2 g; copper sulfate 5mg; distilled water added to 1000ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; agar; Make up to volume with distilled water, sterilize twice at low temperature, 45 min each time, and pack after sterilization.

Embodiment 3

[0028] Example 3, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; 0.5 g; Potassium dihydrogen phosphate 0.2 g; L-cysteine 0.1 g; add distilled water to 1000ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; agar; After uniform filtration, distilled water to volume, low-temperature sterilization twice, 45 min each time, after sterilization, aliquot.

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Abstract

The invention discloses a staphylococcus cohnii selective medium and a preparation method thereof. The staphylococcus cohnii selective medium is characterized in that the 1000 ml of selective medium comprises the following components by weight: 15g of malt extract, 150ml of egg protein liquid, 5g of sodium chloride, 0.5g of magnesium citrate, 15g of agar, 8-12 mg of coetsoidin, 0.5g of acetovanillone and 0.2g of monopotassium phosphate, which are prepared to 1000 ml by adding distilled water. The optical preparation method comprises the following steps of: dissolving the malt extract, the egg protein liquid, sodium chloride, magnesium citrate, the agar, the coetsoidin, the acetovanillone, the monopotassium phosphate, L-cysteine and copper sulfate in lily decocted liquid; and uniformly mixing the components, metering the volume of the mixture, sterilizing, splitting and packaging the mixture. Compared with the prior art, the detection is reliable, and the separation rate of the staphylococcus lentus is high.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a selective culture medium for Staphylococcus koshii and a preparation method thereof. Background technique [0002] Staphylococcus cohnii (Staphylococcus cohnii) belongs to the Staphylococcus genus (Staphylococcus), which is a low-virulence opportunistic pathogen. Symptoms after infection are not typical, and multi-drug resistance is present, which brings certain benefits to clinical diagnosis and treatment. Difficult, efficient and reliable inspection methods are inevitable. [0003] At present, the identification of Staphylococcus koridi is mainly through the API Staph Staphylococcus and micrococcus identification system, but based on research and drug tests, there is a lack of a dedicated selective medium for Staphylococcus koridi. [0004] Manitol Salt Agar is the commonly used selective culture medium for Staphylococcus aureus, which is used for the selective isolation and c...

Claims

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Application Information

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IPC IPC(8): C12Q1/14C12R1/44
Inventor 陈淑香
Owner 陈淑香
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