Lactobacillus casei ghost, preparation method and application thereof
A technology of Lactobacillus casei and Lactobacillus, applied in the biological field, can solve the problems of not being able to guarantee 100% complete lysis of bacteria, spreading pathogens and infections, and not being able to be popularized and applied, so as to achieve heavy workload, strengthen immunogenicity, and improve immunity horizontal effect
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Embodiment 1
[0039] The preparation of embodiment 1 Lactobacillus casei bacteria shadow and the identification of basic characteristics
[0040] 1. Materials and methods
[0041] 1.1 Test material
[0042] 1.1.1 Plasmids and strains
[0043] Lactobacillus casei L.casei 393 was donated by NIZO Research Institute in the Netherlands, and Escherichia coli competent JM109 and pMD18-T Simple vectors were purchased from Dalian Bao Biological Engineering Co., Ltd.
[0044] 1.1.2 Molecular biology reagents
[0045] Ex Taq DNA polymerase, dNTP and restriction endonuclease were purchased from TaKaRa Company, T4 DNA ligase was purchased from NEB Company, plasmid miniprep kit and DNA gel recovery kit were purchased from Axygen Company.
[0046] 1.1.3 Primer synthesis
[0047] Primers were designed using oligo6.0 software, primer 1 and primer 2 were used to amplify the perforin (hocb) gene fragment from the phage genome; primer 3 and primer 4 were used to amplify the lysin (lycb) gene fragment from ...
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