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Bionic three-dimensional tissue engineering scaffold and preparation method thereof

A tissue engineering scaffold, three-dimensional technology, applied in scaffolds, prostheses, medical science, etc., can solve the problem of low drug loading rate, and achieve the effect of improving loading rate and guiding regenerative function

Inactive Publication Date: 2013-04-03
江西欧芮槿生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for emulsion electrospinning, in order to ensure the stability of the electrospun emulsion, the drug loading rate is often low

Method used

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  • Bionic three-dimensional tissue engineering scaffold and preparation method thereof
  • Bionic three-dimensional tissue engineering scaffold and preparation method thereof
  • Bionic three-dimensional tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Dissolve 0.40 g of polycaprolactone (PCL, molecular weight: 60,000) in 3.5 ml of 1,2-dichloromethane and 0.5 ml of xylene, and drop into 20 microliters of Span with a pipette. After the PCL was completely dissolved under magnetic stirring, 250 microliters of active growth factor-rich PRP (containing 5% dextran as a protective agent) was added dropwise. Stir for 45 minutes under magnetic stirring. At the same time, gelatin (GE, molecular weight 100,000), fibroblast growth factor bFGFb, and endothelial cell growth factor VEGF were dissolved in trifluoroethanol to prepare a mixed solution with a total concentration of 12w / v%, wherein the active growth factor accounted for 0.05% of solids.

[0045] (2) Select a single nozzle spinning device, put the mixed solution of the PCL electrospun emulsion containing PRP, gelatin and active factor in step (1) into two different infusion channels respectively, and adjust the feeding rate of the solution to 20ul / min, the distance...

Embodiment 2

[0048] (1) Dissolve 0.40 g of polyglycolide (PLGA, molecular weight: 100,000) in 8 mL of chloroform, and drop into 50 microliters of Span 80 with a pipette. After the PLGA was completely dissolved under magnetic stirring, 250 microliters of PRP (containing 5% dextran as a protective agent) was added dropwise. Stir for 45 minutes under magnetic stirring. At the same time, gelatin (GE, molecular weight 100,000), hyaluronic acid (HA, molecular weight 500,000), fibroblast growth factor bFGFb, and endothelial cell growth factor VEGF were dissolved in a mixed solvent with a volume ratio of DMF and water of 1:1. The fixed hyaluronic acid concentration is 1%, the mass ratio of hyaluronic acid and gelatin is 100:20, and the active molecule accounts for 0.08% of the solid content.

[0049] (2) Select a single-nozzle spinning device, put the mixed solution of the PLGA electrospun emulsion containing PRP and gelatin / hyaluronic acid / active factor in step (1) into two different infusion ch...

Embodiment 3

[0052] (1) Polyethylene lactide (PLGA, molecular weight 80,000) was dissolved in 8 mL of chloroform, and 50 microliters of Span 80 was dropped into it with a pipette gun. After the PLGA was completely dissolved under magnetic stirring, 250 microliters of PRP (containing 5% dextran as a protective agent) was added dropwise. Stir for 45 minutes under magnetic stirring. At the same time, gelatin (GE, molecular weight 100,000) was dissolved in a mixed solvent with a volume ratio of water and ethanol of 9:1 to prepare a mixed solution with a total concentration of 15w / v%.

[0053](2) select double-nozzle spinning device for use, the PLGA solution of step (1) and the GE mixed solution containing active molecules are respectively packed in two different infusion pathways, and the feeding rate of the adjustment solution is 15ul / min, and the spinning The distance between the head and the grounded collector is 12cm; the ambient temperature is 25°C; the electrostatic voltage of PLGA ele...

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Abstract

The invention discloses a bionic three-dimensional tissue engineering scaffold, which is formed by high molecular fibrous membrane-loaded active growth factors, or active growth factors loaded on a composite scaffold formed by a high molecular fibrous membrane and a macropore spongy layer. With the adoption of the bionic three-dimensional tissue engineering scaffold, the problem that the concentration of active molecules loaded with an emulsion electricity texture fibrous membrane is low; the emulsion electricity texture fibrous membrane is combined with a macropore spongy or a mixed electricity texture process, so that the load rate of the active factors can be greatly improved; parts of factors are retained in the fiber through an emulsion electricity texture core-shell structure, so that the effective control of releasing time is realized, and a repairing process is monitored for a long time; and the introduction of active molecules in the scaffold plays guiding and promoting functions for proliferating regenerative cells, directionally differentiating, migrating and adhering cells, and capturing stem cells to introduce regenerative functions of newly born tissues, so that a new path is provided for development of regenerative medicine industries.

Description

technical field [0001] The invention relates to biological materials, in particular to a biomimetic three-dimensional tissue engineering scaffold with long-term inducing regeneration effect on tissues and a preparation method thereof. Background technique [0002] The repair of complex and large-area wounds not only depends on scaffold materials, but also requires growth factors to regulate the formation of special structures in the process of cell differentiation and tissue development. Due to the instability of proteins and genes, traditional protein or gene pharmaceutical dosage forms, such as injections, are not conducive to maintaining the structure and activity of proteins and genes for a long time. At the same time, growth factors can easily lose their biological activity in the presence of water and enzymes. Therefore, the direct use of growth factors often faces the dilemma of short biological activity retention time, and the expected biological efficiency cannot b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/82A61L27/26A61L27/54
Inventor 聂华荣汪泱郭菲易应萍邓志锋
Owner 江西欧芮槿生物科技有限公司
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