Pure chicken powder and preparation method and application thereof
The technology of pure chicken powder and chicken is applied in the field of preparation of flavor base materials, which can solve the problems of weak flavor intensity and the like, and achieve the effects of improving strong flavor, safe operation and good thermal stability.
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Embodiment 1
[0023] (1) Take chicken breast as raw material, add 0.8 times the weight of water, pass through a colloid mill, add flavored protease to hydrolyze until the degree of hydrolysis of chicken protein is 15%, keep at 80°C for 30 minutes, and obtain chicken protein enzymatic hydrolyzate;
[0024] (2) Enzymolysis and concentration of chicken protein to a solid content of 30% to obtain an enzymolysis concentrate;
[0025] (3) Add 3% glutamic acid and 0.1% pepsin to the enzymolysis concentrated solution, keep it at 40°C for 3 hours, keep at 80°C for 30 minutes to inactivate the enzyme, centrifuge at 6000g for 30 minutes, and obtain the enzymolysis reaction liquid;
[0026] (4) Pass the enzymatic hydrolysis reaction solution through a 50kDa ultrafiltration membrane to remove large molecular weight polypeptides and proteins to obtain the permeate;
[0027] (5) Spray-dry or freeze-dry the permeate to obtain pure chicken powder.
[0028]
Embodiment 2
[0030] (1) Take chicken breast meat as raw material, add 5 times the weight of water, pass through a colloid mill, add alkaline protease to hydrolyze until the degree of hydrolysis of chicken protein is 20%, keep at 95°C for 10 minutes, and obtain chicken protein enzymatic hydrolysis solution;
[0031] (2) Enzymolysis and concentration of chicken protein to a solid content of 40% to obtain an enzymolysis concentrate;
[0032] (3) Add 5% aspartic acid by weight of the concentrate to the enzymatic hydrolysis concentrate, add 0.3% chymotrypsin by weight of the concentrate, incubate at 60°C for 1h, keep at 95°C for 10min to inactivate the enzyme, centrifuge at 8000g for 15min to obtain Enzymolysis reaction solution;
[0033] (4) Pass the enzymatic hydrolysis reaction solution through a 100kDa ultrafiltration membrane to remove large molecular weight polypeptides and proteins to obtain the permeate;
[0034] (5) Spray-dry or freeze-dry the permeate to obtain pure chicken powder. ...
Embodiment 3
[0037] (1) Take chicken breast meat as raw material, add 2 times the weight of water, pass through a colloid mill, add trypsin to hydrolyze until the degree of hydrolysis of chicken protein is 18%, keep at 90°C for 20 minutes, and obtain chicken protein enzymatic hydrolyzate;
[0038] (2) Enzymolysis and concentration of chicken protein to a solid content of 35% to obtain an enzymolysis concentrate;
[0039] (3) Add 4% glycine by weight of the concentrated solution to the enzymatic hydrolysis concentrated solution, add 0.2% pepsin by weight of the concentrated solution, incubate at 50°C for 2 hours, keep at 90°C for 20 minutes to inactivate the enzyme, and centrifuge at 7000g for 20 minutes to obtain the enzymatic hydrolysis reaction solution;
[0040] (4) Pass the enzymatic hydrolysis reaction solution through a 50kDa ultrafiltration membrane to remove large molecular weight polypeptides and proteins to obtain the permeate;
[0041] (5) Spray-dry or freeze-dry the permeate to...
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