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Method for producing polypeptide antibiotics nosiheptide through fermentation

A technology of polypeptide antibiotics and nosiheptide, which is applied in the fields of fermentation engineering, pharmaceutical engineering, and industrial microorganism technology, can solve the problems of low fermentation yield and affect economic benefits, and achieve the effect of maintaining vitality, high titer, and not easy to fall.

Active Publication Date: 2013-12-25
江西兴鼎科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production level of nosiheptide fermentation in my country is relatively low compared with foreign manufacturers, which affects economic benefits

Method used

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  • Method for producing polypeptide antibiotics nosiheptide through fermentation

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Comparison scheme
Effect test

Embodiment 1

[0035] A method for producing polypeptide antibiotic nosiheptide by fermentation method, the technical scheme is as follows:

[0036] (1) Slant culture: Inoculate CPCC600041-viable Streptomyces purchased from Beijing Bei Nachuanglian Biotechnology Research Institute, the strain preservation number is CPCC 600041, into the nutrient medium, and carry out slant culture to obtain spore-producing slant;

[0037] (2) Seed culture: inoculate the slant spores into the seed culture medium, carry out seed culture, and obtain the seed liquid, the solid content of the seed liquid accounts for 20% of the total amount of the seed liquid;

[0038] (3) Fermentation culture: inoculate the seed liquid into the fermentation medium at 10% inoculum amount, and carry out fermentation culture. Ferment to obtain a brown fermented liquid. After pretreatment, the filtered mycelium is extracted with alcohol and n-butanol to obtain pure nosiheptide.

[0039] When cultivating on a slant, the composition ...

Embodiment 2

[0043] A method for producing polypeptide antibiotic nosiheptide by fermentation method, the technical scheme is as follows:

[0044] (1) Slant culture: Inoculate CPCC600041-viable Streptomyces purchased from Beijing Bei Nachuanglian Biotechnology Research Institute, the strain preservation number is CPCC 600041, into the nutrient medium, and carry out slant culture to obtain spore-producing slant;

[0045](2) Seed culture: Inoculate the slant spores into the seed culture medium, carry out seed culture, and obtain the seed liquid, the solid content of the seed liquid accounts for 25% of the total amount of the seed liquid;

[0046] (3) Fermentation culture: inoculate the seed liquid into the fermentation medium at 10% inoculum amount, and carry out fermentation culture. Ferment to obtain a brown fermented liquid. After pretreatment, the filtered mycelium is extracted with alcohol and n-butanol to obtain pure nosiheptide.

[0047] When cultivating on a slant, the composition o...

Embodiment 3

[0051] A method for producing polypeptide antibiotic nosiheptide by fermentation method, the technical scheme is as follows:

[0052] (1) Slant culture: Inoculate CPCC600041-viable Streptomyces purchased from Beijing Bei Nachuanglian Biotechnology Research Institute, the strain preservation number is CPCC 600041, into the nutrient medium, and carry out slant culture to obtain spore-producing slant;

[0053] (2) Seed culture: Inoculate the slant spores into the seed culture medium, carry out seed culture, and obtain the seed liquid, the solid content of the seed liquid accounts for 30% of the total amount of the seed liquid;

[0054] (3) Fermentation culture: inoculate the seed liquid into the fermentation medium at 10% inoculum amount, and carry out fermentation culture. Ferment to obtain a brown fermented liquid. After pretreatment, the filtered mycelium is extracted with alcohol and n-butanol to obtain pure nosiheptide.

[0055] When cultivating on a slant, the composition ...

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Abstract

The invention discloses a method for producing polypeptide antibiotics nosiheptide through fermentation. The method comprises the following steps of: inoculating streptomyces actuosus to a slant culture medium for carrying out slant culture; inoculating an obtained slant with spores to a seed bottle to culture a seed solution; inoculating the seed solution to a fermentation culture medium for carrying out fermentation culture according to an inoculation quantity of 10 wt%; and further treating the fermented fermentation liquor to obtain nosiheptide crystals. The nosiheptide is produced by adopting a fermentation method. The method is simple and easy and is low in production cost. The polypeptide antibiotics nosiheptide produced by adopting the method can be used as a safe, high-efficiency and environmental-friendly novel feed additive.

Description

technical field [0001] The invention relates to a fermentation method for producing polypeptide antibiotic nosiheptide, which belongs to the fields of industrial microorganism technology, fermentation engineering and pharmaceutical engineering. Background technique [0002] Nosiheptide, also known as Nosiceptide, is a new type of green and environmentally friendly feed additive. It is a special antibiotic for animals. It is produced by active streptomyces ( Streptomyces actuosus ) Sulfur-containing polypeptide antibiotics produced by fermentation. It has a strong inhibitory effect on Gram-positive bacteria, inhibits the growth of Gram-positive bacteria by inhibiting the elongation factors Tu and G in the translation process, and also inhibits the synthesis of guanosine tri- and tetraphosphatases. Nosiheptide is a yellow or yellow-green crystal, insoluble in water, with a melting point of 310-320°C and a molecular formula of C 51 h 43 N 13 o 12 S 6 , The element composi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12R1/465
Inventor 韩风华李国栋邹君彪邓健黄苓
Owner 江西兴鼎科技有限公司
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