Method for producing polypeptide antibiotics nosiheptide through fermentation
A technology of polypeptide antibiotics and fermentation method, which is applied in the field of production of polypeptide antibiotics nosiheptide by fermentation method, can solve the problems of low fermentation yield and impact on economic benefits, and achieves the effects of maintaining vitality, high titer, and not easy to fall.
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Embodiment 1
[0035] A method for producing polypeptide antibiotic nosiheptide by fermentation method, the technical scheme is as follows:
[0036] (1) Slant culture: Inoculate CPCC600041-viable Streptomyces purchased from Beijing Bei Nachuanglian Biotechnology Research Institute, the strain preservation number is CPCC 600041, into the nutrient medium, and carry out slant culture to obtain spore-producing slant;
[0037] (2) Seed culture: inoculate the slant spores into the seed culture medium, carry out seed culture, and obtain the seed liquid, the solid content of the seed liquid accounts for 20% of the total amount of the seed liquid;
[0038] (3) Fermentation culture: inoculate the seed liquid into the fermentation medium at 10% inoculum amount, and carry out fermentation culture. Ferment to obtain a brown fermented liquid. After pretreatment, the filtered mycelium is extracted with alcohol and n-butanol to obtain pure nosiheptide.
[0039] When cultivating on a slant, the composition ...
Embodiment 2
[0043] A method for producing polypeptide antibiotic nosiheptide by fermentation method, the technical scheme is as follows:
[0044] (1) Slant culture: Inoculate CPCC600041-viable Streptomyces purchased from Beijing Bei Nachuanglian Biotechnology Research Institute, the strain preservation number is CPCC 600041, into the nutrient medium, and carry out slant culture to obtain spore-producing slant;
[0045](2) Seed culture: Inoculate the slant spores into the seed culture medium, carry out seed culture, and obtain the seed liquid, the solid content of the seed liquid accounts for 25% of the total amount of the seed liquid;
[0046] (3) Fermentation culture: inoculate the seed liquid into the fermentation medium at 10% inoculum amount, and carry out fermentation culture. Ferment to obtain a brown fermented liquid. After pretreatment, the filtered mycelium is extracted with alcohol and n-butanol to obtain pure nosiheptide.
[0047] When cultivating on a slant, the composition o...
Embodiment 3
[0051] A method for producing polypeptide antibiotic nosiheptide by fermentation method, the technical scheme is as follows:
[0052] (1) Slant culture: Inoculate CPCC600041-viable Streptomyces purchased from Beijing Bei Nachuanglian Biotechnology Research Institute, the strain preservation number is CPCC 600041, into the nutrient medium, and carry out slant culture to obtain spore-producing slant;
[0053] (2) Seed culture: Inoculate the slant spores into the seed culture medium, carry out seed culture, and obtain the seed liquid, the solid content of the seed liquid accounts for 30% of the total amount of the seed liquid;
[0054] (3) Fermentation culture: inoculate the seed liquid into the fermentation medium at 10% inoculum amount, and carry out fermentation culture. Ferment to obtain a brown fermented liquid. After pretreatment, the filtered mycelium is extracted with alcohol and n-butanol to obtain pure nosiheptide.
[0055] When cultivating on a slant, the composition ...
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