Primer and reagent kit capable of being used for detecting southern rice black-streaked dwarf virus (SRBSDV) as well as quantitative detection method of SRBSDV

Abstract

The invention discloses a primer and a reagent kit capable of being used for detecting southern rice black-streaked dwarf virus (SRBSDV) and also discloses a method for quantitatively detecting the SRBSDV by the reagent kit. According to the method, firstly, the primer is synthesized; then, an RNA (ribonucleic acid) extraction reagent kit is utilized for extracting RNA of the primer from samples; the RNA is used as a template, in addition, a cDNA (complementary deoxyribonucleic acid) synthetic reaction system is utilized for synthesizing cDNA, then, positive standard samples are prepared and are subjected to real-time fluorescence quantification PCR (polymerase chain reaction) procedure drawing, and a standard curve of circulation number-initial concentration logarithmic values with fluorescence signals reaching the threshold values is obtained; and finally, a real-time fluorescence quantification RT-PCR (reverse transcription-polymerase chain reaction) system is prepared by using the synthesized cDNA as the template, the amplified reaction is carried out through the real-time fluorescence quantification RT-PCR procedure, and the initial concentration value of the SRBSDV in the samples is measured according to the collected fluorescence signals and the standard curve. The method has the advantages that the precision is high, the stability is good, the repeatability is good, the specificity is high, the sensitivity is high, and the like.

Description

technical field The invention belongs to the technical field of plant virus molecular biology detection and identification, and in particular relates to a primer, a kit and a detection method for plant virus detection. Background technique Southern rice black-streaked dwarf virus (SRBSDV) is a new virus that seriously harms rice this year. Since 2009, disasters have broken out in the main rice-growing areas in southern my country. In 2010 alone, the southern rice black-streaked dwarf virus disease occurred in an area of ​​more than 14 million mu, seriously threatening the safe production of rice in my country. Southern rice black-streaked dwarfvirus (SRBSDV) belongs to the Reoviridae family Fijivirus (Fijivirus), the virion is spherical, and the genome consists of 10 double-stranded RNA (dsRNA). Transmitted in a persistent manner by the white-backed planthopper (Sogatella furcifera Horvath). Symptoms of rice infestation are dwarf plants, dark green leaves, high tillers, ...

AI Technical Summary

Problems solved by technology

However, none of the existing technologies can quantitatively detect the viral content of SRBSDV

Images