Method for building small pig model for research on lower limb deep venous thrombus formation and pulmonary embolism

Abstract

The invention belongs to the field of animal medical models and particularly relates to a method for building a small pig model for researches on lower limb deep venous thrombus formation and pulmonary embolism. The method is characterized in that the method comprises the steps of firstly forming small pig posterior limb deep venous thrombus, cutting a small pig anterior limb cephalic vein open for intubation, injecting the in-vitro prepared thrombus into a superior vena cava and delivering the thrombus to a pulmonary artery through cardiac circulation to cause pulmonary artery embolism to build the small pig model for researches on lower limb deep venous thrombus formation and pulmonary embolism, wherein aminomethylbenzoic acid injection in appropriate proportion being 1: (15-20) is added during the preparation of the in-vitro thrombus. By building the small pig model for researches on lower limb deep venous thrombus formation and pulmonary embolism, a good animal model can be provided for pathophysiologic researches on diseases, clinical drug researches and surgical practices. The method provided by the invention has the advantages of simple anesthesia operation, clear effect, simple and rapid surgical operation, high pulmonary embolism formation success rate, low fatality rate, convenient inspection means and the like.

Description

technical field [0001] The invention relates to a medical animal model, in particular to a method for establishing a miniature pig model of lower extremity deep vein thrombosis combined with pulmonary embolism. Background technique [0002] In the research on the treatment of deep vein thrombosis of lower extremities and pulmonary embolism, nuclide pulmonary perfusion imaging, enhanced spiral CT and digital subtraction pulmonary angiography need to be carried out through animal models, and the pathological examination of animal models is used as the standard to compare the three inspection methods sensitivity and specificity. Since miniature pigs are used to simulate deep venous thrombosis combined with pulmonary embolism, which is closer to the natural clinical process of humans, the most commonly used animal model for lower extremity deep venous thrombosis and pulmonary embolism is experimental miniature pigs. [0003] According to existing literature, the application of ...

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Problems solved by technology

[0005] At present, there are more and more clinical cases of deep venous thrombosis of upper and lower extremities combined with pulmonary embolism. Nuclide pulmonary perfusion imaging, enhanced spiral CT and digital subtraction pulmonary angiography pathological examinations are performed only through animal models of venous thrombosis and pulmonary embolism. Sensitivity and specificity are not enough, and it is urgent to establish an animal model of lower extremity deep vein thrombosis combined with pulmonary embolism to accurately analyze the pathological characteristics of this case