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Process for preparing snake venom hemostatic medicine

A technology for hemostatic drugs and snake venom is applied in the field of preparation of snake venom hemostatic drugs, and can solve the problems of long time for immediate hemostasis and the like

Inactive Publication Date: 2003-08-06
成都诺迪康生物制药有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the only hemostatic drug made from snake venom is the Swiss Lihemostatic technology. The clotting time of this technology product is 2 minutes and 05 seconds, which is still too long for special cases that require immediate hemostasis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The concrete steps of preparation snake venom hemostatic medicine are as follows:

[0020] 1. Dissolve 2.0g of snake venom in 50ml of deionized water,

[0021] 2. Stand at 10°C for 1 hour,

[0022] 3. Centrifuge in a centrifuge with a centrifugal speed of 10,000 rpm and a temperature of -20°C.

[0023] 4. Filter the supernatant obtained by centrifugation through a 10KD ultrafiltration membrane,

[0024] 5. Through column chromatography, the filler is Sephadex C25, and the ammonium acetate solution with pH=5.6 and concentration=0.2mol / L is used for gradient elution, and the elution rate is 30ml / hour.

[0025] 6. Collect activity peaks, concentrate and desalt, the concentration of gelatin is 3%,

[0026] 7. Freeze-dry at -4°C for 1 hour to obtain the purified snake venom hemostatic drug with a yield of 2%.

[0027] The snake venom hemostatic drug produced by the above preparation method is white freeze-dried powder in appearance, has no special smell and is easily sol...

Embodiment 2

[0029] The concrete steps of preparation snake venom hemostatic medicine are as follows:

[0030] 1. Dissolve 100g of snake venom in 2500ml of deionized water;

[0031] 2. Stand at 10°C for 1 hour;

[0032] 3. Centrifuge in a centrifuge with a centrifugal speed of 10,000 rpm and a temperature of -20°C;

[0033] 4. Filter the supernatant obtained by centrifugation through a 10KD ultrafiltration membrane;

[0034] 5. Through column chromatography, the filler is Sephadex C25, and the ammonium acetate solution with pH=5.6 and concentration=0.2mol / L is used for gradient elution, and the elution rate is 30ml / hour;

[0035] 5. Collect activity peaks, concentrate and desalt, and the concentration of gelatin is 3%;

[0036] 6. Freeze-dry at -4°C for 1 hour to obtain the purified snake venom hemostatic drug with a yield of 2%.

[0037] The snake venom hemostatic drug produced by the above preparation method is white freeze-dried powder in appearance, has no special smell and is easi...

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PUM

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Abstract

The preparation method of snake venom hemostat includes the following steps: 1. dissolving snake venom in deionized water, 2. high-speed freezing and centrifugalization; 3. taking supernatant fluid and making it pass through 10 KD ultrafiltration membrane; 4. column chromatography; 5. graident elution by using NH4Ac solution; 6. collecting activity peak; and 7. freeze-drying so as to obtain the invented product which can be used in several links of blood coagulation process, can be used for preventing and controlling various hemorrhages, and can be used for intravenous injection.

Description

technical field [0001] The invention relates to a preparation method of snake venom hemostatic medicine. Background technique [0002] Existing human body hemostatic drugs, commonly used have Baiyao, thrombin etc., Baiyao can be taken orally or externally; Thrombin can only be used externally but not orally. [0003] At present, the only one that uses snake venom to make hemostatic medicine is the Swiss Lihemostatic technology. The clotting time of this technology product is 2 minutes and 05 seconds, which is still too long for special cases that require immediate hemostasis. Invention content: [0004] In view of the current status of blood coagulation drugs, the purpose of this invention is to study a human body hemostatic drug that has a faster coagulation time and can be injected intravenously. [0005] The preparation method of snake venom hemostatic medicine that the present invention relates to has reached above-mentioned purpose, and its preparation steps are as f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/583A61P7/04
Inventor 罗勇刚叶立人
Owner 成都诺迪康生物制药有限公司
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