Urea isotopic abundance rapid detection method based on Raman spectrum

Abstract

The invention relates to a urea isotopic abundance rapid detection method based on Raman spectrum. A traditional detection method is slow in speed and complex in operation. 15N double-tagging urea to be tested is placed at the center of a glass slide directly and pressed slightly by cover glass to enable the 15N double-tagging urea to be smooth in surface so as to obtain a sample. The sample is fixed on an objective table below a lens of a microscope of a Raman spectrometer, magnification times of the microscope is adjusted so as to enable the sample to make imaging clearly in an eyepiece field, and a laser is used for acquiring the Raman spectrum in a range of 3500cm<-1>-34.4cm<-1>. Finally, on the basis of the Raman spectrum of the sample in the range of 3500cm<-1>-34.4cm<-1>, a specific fingerprint waveband is screened out by adopting the successive projections algorithm, an intensity value of the Raman spectrum at the waveband is obtained, and isotopic abundance is calculated by using the intensity value of the Raman spectrum. The urea isotopic abundance rapid detection method based on the Raman spectrum is convenient to use and can measure plant 15N tracer isotope abundance rapidly and effectively.

Description

technical field [0001] The invention belongs to the technical field of information detection and relates to a 15 Raman spectroscopic rapid detection method of N double-labeled urea isotope abundance for rapid measurement of double-labeled urea 15 N isotopic abundance. Background technique [0002] Nitrogen is an essential nutrient element for plants and the first of the three elements of crop fertilization. Nitrogen fertilizer accounts for about 60% of my country's chemical fertilizer consumption. At present, my country's 7% of the world's arable land consumes 35% of the world's nitrogen fertilizer, and the excessive application of nitrogen fertilizer has brought great harm to my country's agricultural production and ecological environment. Every year, nearly 10 million tons of nitrogen are lost due to unreasonable fertilization in China, and the direct economic loss is about 30 billion yuan. Moreover, due to the low utilization rate of nitrogen fertilizer, about 60% ente...

AI Technical Summary

Problems solved by technology

At the same time, Raman spectroscopy can effectively overcome the traditional 15 The N isotope analysis method is expensive to measure, time-consuming to analyze and test, the number of samples is too small, and the detection cycle is long.

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